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. 2024 Feb;4(2):e990.
doi: 10.1002/cpz1.990.

Induction of Paraquat-Mediated Parkinsonian Phenotype in Zebrafish

V S Harini  1 , Raghunath Marimuthu  1 , M S Ananthakrishna Tantry  1 , Kirankumar Santhakumar  1
Affiliations

Induction of Paraquat-Mediated Parkinsonian Phenotype in Zebrafish

V S Harini et al. Curr Protoc. 2024 Feb.

Abstract

Paraquat (PQ) is a well-known neurotoxin closely associated with neurodegenerative Parkinson's disease (PD). Zebrafish are utilized as a model for PD research because of their well-defined neuropathology and locomotor behavior. Here, we highlight protocols for inducing PD using PQ and analyzing locomotor activity in adult zebrafish. Basic Protocol 1 details the treatment of adult male zebrafish with 60 mg/kg PQ via intraperitoneal injection to induce a PD-like phenotype, followed by the steps to perform a locomotor assay. Basic Protocol 2 provides step-by-step guidance for processing the acquired videos in ToxTrac software to understand the locomotor parameters of 0.9% saline- and 60 mg/kg PQ-injected adult zebrafish. The simplicity of the treatment strategy, low-cost video acquisition setup, and free video processing make these protocols accessible without prior experience. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Development of Parkinson's disease features in adult zebrafish Basic Protocol 2: ToxTrac analysis for locomotor assay.

Keywords: Parkinson's disease; ToxTrac; locomotor behavior; paraquat; zebrafish.

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References

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