Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2024 Feb 6:2024:3355239.
doi: 10.1155/2024/3355239. eCollection 2024.

Current Strategies for Tracheal Decellularization: A Systematic Review

Dhihintia Jiwangga  1 , Ferdiansyah Mahyudin  2 , Gondo Mastutik  3 , Juliana  4 , Estya Nadya Meitavany  5
Affiliations
Review

Current Strategies for Tracheal Decellularization: A Systematic Review

Dhihintia Jiwangga et al. Int J Biomater. .

Abstract

The process of decellularization is crucial for producing a substitute for the absent tracheal segment, and the choice of agents and methods significantly influences the outcomes. This paper aims to systematically review the efficacy of diverse tracheal decellularization agents and methods using the PRISMA flowchart. Inclusion criteria encompassed experimental studies published between 2018 and 2023, written in English, and detailing outcomes related to histopathological anatomy, DNA quantification, ECM evaluation, and biomechanical characteristics. Exclusion criteria involved studies related to 3D printing, biomaterials, and partial decellularization. A comprehensive search on PubMed, NCBI, and ScienceDirect yielded 17 relevant literatures. The integration of various agents and methods has proven effective in the process of tracheal decellularization, highlighting the distinct advantages and drawbacks associated with each agent and method.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Flowchart of the data gathering process.
Figure 2
Figure 2
The chemical reaction for sodium dodecyl sulfate.
See this image and copyright information in PMC

References

    1. Farzanegan R., Feizabadi M., Ghorbani F., et al. An overview of tracheal stenosis research trends and hot topics. Archives of Iranian Medicine . 2017;20(9):598–607. - PubMed
    1. Berg M., Ejnell H., Kovács A., et al. RETRACTED: replacement of a tracheal stenosis with a tissue-engineered human trachea using autologous stem cells: a case report. Tissue Engineering Part A . 2014;20(1-2):389–397. doi: 10.1089/ten.tea.2012.0514. - DOI - PubMed
    1. Hancox Z., Yousaf S., Farzi G. A., et al. Scaffolds for tracheal tissue engineering. In: Mozafari M., Sefat F., Atala A., editors. Handbook of Tissue Engineering Scaffolds: Volume Two . Cambridge, UK: Woodhead Publishing; 2019. pp. 361–391.
    1. White L. J., Taylor A. J., Faulk D. M., et al. The impact of detergents on the tissue decellularization process: a ToF-SIMS study. Acta Biomaterialia . 2017;50:207–219. doi: 10.1016/j.actbio.2016.12.033. - DOI - PMC - PubMed
    1. Hoshiba T., Chen G., Endo C., et al. Decellularized extracellular matrix as an in vitro model to study the comprehensive roles of the ECM in stem cell differentiation. Stem Cells International . 2016;2016:10. doi: 10.1155/2016/6397820.6397820 - DOI - PMC - PubMed

LinkOut - more resources