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. 2024 Feb 14;24(1):85.
doi: 10.1186/s12906-024-04343-2.

The study of wound healing activity of Thespesia populnea L. bark, an approach for accelerating healing through nanoparticles and isolation of main active constituents

Menna B Abdel Halim  1 , Hanaa H Eid  2 , Kadriya S El Deeb  2 , Ghada F Metwally  3 , Marwa A Masoud  4 , Omar A Ahmed-Farid  5 , Hussien M El Messiry  6
Affiliations

The study of wound healing activity of Thespesia populnea L. bark, an approach for accelerating healing through nanoparticles and isolation of main active constituents

Menna B Abdel Halim et al. BMC Complement Med Ther. .

Abstract

The present study provides an evaluation for the wound healing activity of the ethanolic extract of Thespesia populnea L. bark (EBE) and its successive fractions in two doses level (1&2%), designed for determining the most bioactive fraction and the suitable dose. Furthermore, development of the most convenient formulation for these bioactive fractions through either their direct incorporation into hydrogel formulations or incorporation of chitosan-loaded nanoparticles with these bioactive fractions into hydrogel formulations. The highest excision wound healing activity was observed in petroleum ether (Pet-B) followed by ethyl acetate (Etac-B) fractions at the high dose (2%). The most suitable formulation designed for the Etac-B fraction was found to be the chitosan-loaded nanoparticles incorporated in the hydrogel formulation, while the conventional hydrogel formulation was observed to be the highly acceptable formulation for Pet-B fraction. Further phytochemical studies of the bioactive fractions led to the isolation of many compounds of different chemical classes viz; beta-sitosterol and lupeol acetate isolated from the Pet-B, in addition to cyanidin and delphinidin from the Etac-B. Our results revealed that EBE and its bioactive fractions (Pet-B & Etac-B) could be considered as strong wound healers through their anti-oxidant and anti-inflammatory activities, in addition to stimulating collagen synthesis.

Keywords: Anti-oxidant activity; Chitosan-loaded nanoparticles; Collagen amino acids; Thespesia populnea L. bark; Wound healing.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Rheograms of the prepared hydrogel formulations: F1 & F4. F1: hydrogel containing petroleum ether fraction, F4: hydrogel containing chitosan loaded ethyl acetate nanoparticles
Fig. 2
Fig. 2
Photos showing wound contraction activity of the F1-F4 against Panthenol gel, positive control and vehicle (gel base) groups in circular excision wound model on days 2, 5, 7, 11 and 14. F1: hydrogel containing petroleum ether fraction, F2: hydrogel containing chitosan loaded petroleum ether nanoparticles, F3: hydrogel containing ethyl acetate fraction, F4: hydrogel containing chitosan loaded ethyl acetate nanoparticles
Fig. 3
Fig. 3
Photomicrographs of normal skin rats (G1) and wounded skin (stained with H&E) in different groups after 7 & 14 days of circular excision wound model. G2: Untreated group, G3 – G8 Groups treated with vehicle (gel base), Panthenol, formulations F1-F4, respectively. St: standard drug (Panthenol gel)
See this image and copyright information in PMC

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