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. 2024 Feb;14(2):e1575.
doi: 10.1002/ctm2.1575.

Plasma tsRNAs as novel diagnostic biomarkers for renal cell carcinoma

[Article in Catalan]
Meng Ding  1 , Wanqing Zhou  2 , Wenyuan Chen  1   3 , Wenjing Mo  1   3 , Xinyue Guo  1   3 , Yuhang Li  1   3 , Changwei Ji  1 , Guangxiang Liu  1 , Wenli Diao  1 , Hongqian Guo  1
Affiliations

Plasma tsRNAs as novel diagnostic biomarkers for renal cell carcinoma

[Article in Catalan]
Meng Ding et al. Clin Transl Med. 2024 Feb.
No abstract available

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Conflict of interest statement

The authors declare they have no conflicts of interest.

Figures

FIGURE 1
FIGURE 1
Identification of differentially expressed plasma tsRNAs in RCC patients. (A) Heat map of the expression profile of plasma tsRNAs in 5 RCC patients and 5 healthy controls. (B) Scatter plots of differentially expressed plasma tsRNAs. The red dots indicate upregulated tsRNAs, the blue dots are for downregulated tsRNAs, and the grey dots are for nondifferentially expressed tsRNAs. The grouping criteria is fold‐change ≥ 1.5, p < .05. (C) The relative expression levels of 12 plasma tsRNAs in 32 RCC patients and 30 healthy controls using RT‐qPCR. *p < .05; **p < .01; ***p < .001.
FIGURE 2
FIGURE 2
Validation and diagnosis value of differentially expressed plasma tsRNAs in RCC patients. (A) The relative expression levels of 5 plasma tsRNAs in 120 RCC patients and 99 healthy controls using RT‐qPCR. (B) ROC curves for the abilities of 5 individual plasma tsRNAs to discriminate RCC patients from healthy controls in a total of 152 RCC patients and 129 healthy controls. (C) The relative expression levels of tRF‐19‐DRMD5112 in testing set (43 RCC patients and 31 healthy controls) using RT‐qPCR. (D) ROC curve for the ability‐ of plasma tRF‐19‐DRMD5112 level to discriminate RCC patients from healthy controls in testing set. ***p < .001.
FIGURE 3
FIGURE 3
Biology functions of these 5 tsRNAs in RCC. (A) Relative expression levels of the 5 tsRNAs in RCC tissues and paired normal kidney tissues by RT‐qPCR, N = 21. (B) Representative images (Left) and histogram statistics (Right) from EdU assay of RCC cells transfected with individual tsRNA mimics or negative controls, scale bar = 50 μm. (C) Representative images (Left) and histogram statistics (Right) from colony formation assay of RCC cells transfected with individual tsRNA mimics or negative controls, scale bar = 0.4 cm. (D) Representative images (Left) and histogram statistics (Right) from sphere formation assay of RCC cells transfected with individual tsRNA mimics or negative controls, scale bar = 100 μm. (E) Representative images (Left) and histogram statistics (Right) from transwell migration assay of RCC cells transfected with individual tsRNA mimics or negative controls, scale bar = 200 μm. (F) Representative images (Left) and histogram statistics (Right) from transwell invasion assay of RCC cells transfected with individual tsRNA mimics or negative controls, scale bar = 200 μm. Cell experiment was repeated three times independently, *p < .05; **p < .01; ***p < .001.
FIGURE 4
FIGURE 4
Enrichment analysis of predicted target genes of tRF‐28‐87R8WP9I1E0K. (A) Secondary structure prediction of tRF‐28‐87R8WP9I1E0K. (B) GO analysis of the predicted targets of tRF‐28‐87R8WP9I1E0K. (C) Pathway analysis of the predicted targets of tRF‐28‐87R8WP9I1E0K.
See this image and copyright information in PMC

References

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