doi: 10.1016/0378-1119(79)90082-9.
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells
- PMID: 383576
- DOI: 10.1016/0378-1119(79)90082-9
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Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells
Gene.
1979 May.
Abstract
Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment. With 24-h-old competent cells we obtained routinely 2 . 10(7) transformants per microgram of pBR322 DNA, and transformed over 20% of viable cells.
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