Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Mar;56(3):279-287.
doi: 10.1002/lsm.23761. Epub 2024 Feb 15.

Time-resolved fluorescence and diffuse reflectance for lung squamous carcinoma margin detection

Sarah Costa  1 , Qiyin Fang  2 , Thomas Farrell  3 , Erica Dao  1 , Michael Farquharson  4
Affiliations

Time-resolved fluorescence and diffuse reflectance for lung squamous carcinoma margin detection

Sarah Costa et al. Lasers Surg Med. 2024 Mar.

Abstract

Objectives: A major challenge in non-small cell lung cancer surgery is the occurrence of positive tumor margins. This may lead to the need for additional surgeries and has been linked to poor patient prognosis. This study aims to develop an in vivo surgical tool that can differentiate cancerous from noncancerous lung tissue at the margin.

Methods: A time-resolved fluorescence and diffuse reflectance bimodal device was used to measure the lifetime, spectra, and intensities of endogenous fluorophores as well as optical properties of lung tissue. The tumor and fibrotic tissue data, each containing 36 samples, was obtained from patients who underwent surgical removal of lung tissue after being diagnosed with squamous carcinoma but before any other treatment was administered. The normal lung tissue data were obtained from nine normal tissue samples.

Results: The results show a statistically significant difference between cancerous and noncancerous tissue. The results also show a difference in metabolic related optical properties between fibrotic and normal lung tissue samples.

Conclusions: This work demonstrates the feasibility of a device that can differentiate cancerous and noncancerous lung tissue for patients diagnosed with squamous cell carcinoma.

Keywords: absorption coefficient; diffuse reflectance; fluorescence intensity; fluorescence lifetime; optical properties; optical spectroscopy; scattering coefficient; time-resolved fluorescence.

PubMed Disclaimer

References

REFERENCES

    1. Lung Cancer Statistics 2020. Canadian Cancer Society; 2020. Accessed March 13, 2023. https://cancer.ca/en/cancer-information/cancer-types/lung/statistics
    1. Handy JR, Asaph JW, Skokan L, Reed CE, Koh S, Brooks G, et al. What happens to patients undergoing lung cancer surgery? Chest. 2002;122(1):21-30.
    1. Perez-Moreno P, Brambilla E, Thomas R, Soria JC. Squamous cell carcinoma of the lung: molecular subtypes and therapeutic opportunities. Clin Cancer Res. 2012;18(9):2443-2451.
    1. Harada H, Okada M, Sakamoto T, Matsuoka H, Tsubota N. Functional advantage after radical segmentectomy versus lobectomy for lung cancer. Ann Thorac Surg. 2005;80(6):2041-2045.
    1. Little AG, Rusch VW, Bonner JA, Gaspar LE, Green MR, Webb WR, et al. Patterns of surgical care of lung cancer patients. Ann Thorac Surg. 2005;80(6):2051-2056.

Publication types

MeSH terms

LinkOut - more resources