Correction: Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles

Abstract

[This corrects the article DOI: 10.1371/journal.pone.0137820.].

Fig 4. Cbf11 interacts genetically with the Pka1 pathway.

(A) 10-fold serial dilutions of cultures with the indicated genotypes were spotted on YES plates and grown for 2 days. The slow growth phenotype of Δcbf11 cells is moderately suppressed by the deletion of pka1. (B) Cells growing exponentially in YES were fixed and stained with DAPI. Nuclear integrity defects of Δcbf11 cells (marked with ‘#’) are diminished by deletion of pka1. The DAPI signal was overlaid with the corresponding DIC image to visualize cell contours. Scale bar 10 μm. (C) Calcofluor staining documents that the occurrence of Δcbf11-associated septation defects (e.g., single cells with multiple septa) is decreased in the double mutant with Δpka1. Multicellular filaments are marked by arrows. Scale bar 10 μm. (D) Flow cytometry analysis of DNA content in fixed, propidium iodide-stained cells grown to the exponential phase in YES. Deletion of cbf11 results in aberrant DNA content distribution, which is, in part, corrected by deletion of pka1. Fractions of cells with <2C, 2C, and >2C DNA content are indicated in the histograms. (E) The length of fully septated cells from (C) was measured. Deletion of pka1 has marked influence on the length of Δcbf11 cells. Each dot represents a single cell measurement; median length and n values are indicated above each distribution; WT median value is indicated as a black horizontal line. Data points corresponding to short, multicellular filaments and their n values are shown in blue.