Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Feb 14;4(2):100504.
doi: 10.1016/j.xgen.2024.100504.

Every repeat is unique: Exploring the genomic impact of human L1 retrotransposons at locus-specific resolution

Stephanie Workman  1 , Sandra R Richardson  2
Affiliations

Every repeat is unique: Exploring the genomic impact of human L1 retrotransposons at locus-specific resolution

Stephanie Workman et al. Cell Genom. .

Abstract

Fully understanding the impact of the human retrotransposon L1 requires that each of ∼500,000 L1 copies be evaluated as a potentially unique genomic entity. In this issue of Cell Genomics, Lanciano et al.1 strive toward this goal, illuminating the reciprocal regulatory influence between individual L1s and their genomic integration sites.

PubMed Disclaimer

Conflict of interest statement

Declaration of interests The authors declare no competing interests.

Figures

Figure 1
Figure 1
Illustration of locus- and cell-specific L1 DNA methylation with diverse epigenomic and transcriptomic implications (A) Bisulfite ATLAS -seq (bs-ATLAS-seq) generates locus-specific methylation of the first 15 CpG sites within the L1 5′ UTR. (B) Application of bs-ATLAS-seq across 12 cell lines reveals that young and old L1 families exhibit cell-type-specific methylation levels. Young L1s were hypermethylated in all cell lines with the exception of embryonal lineages. (C) Analysis of methylation at heterozygous L1 insertion sites shows L1-driven methylation changes in the surrounding genome. (D) Transcription factors (TFs) display enrichment across differentially methylated L1 sites in a cell-type-specific manner.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Lanciano S., Philippe C., Sarkar A., Pratella D., Domrane C., Doucet A.J., Essen D.v., Saccani S., Ferry L., Defossez P.-A., et al. Locus-level L1 DNA methylation profiling reveals the epigenetic and transcriptional interplay between L1s and their integration sites. Cell Genom. 2024;4 - PubMed
    1. Lander E.S., Linton L.M., Birren B., Nusbaum C., Zody M.C., Baldwin J., Devon K., Dewar K., Doyle M., FitzHugh W., et al. Initial sequencing and analysis of the human genome. Nature. 2001;409:860–921. doi: 10.1038/35057062. - DOI - PubMed
    1. Luqman-Fatah A., Miyoshi T. Human LINE-1 retrotransposons: impacts on the genome and regulation by host factors. Genes Genet. Syst. 2023;98:121–154. doi: 10.1266/ggs.22-00038. - DOI - PubMed
    1. Kazazian H.H., Jr., Moran J.V. Mobile DNA in Health and Disease. N. Engl. J. Med. 2017;377:361–370. doi: 10.1056/NEJMra1510092. - DOI - PMC - PubMed
    1. Faulkner G.J., Billon V. L1 retrotransposition in the soma: a field jumping ahead. Mob. DNA. 2018;9:22. doi: 10.1186/s13100-018-0128-1. - DOI - PMC - PubMed