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. 2024 Feb 1:15:1352764.
doi: 10.3389/fgene.2024.1352764. eCollection 2024.

Advancing precision in histocompatibility and immunogenetics: a comprehensive review of the UCLA exchange program

Qiuheng Zhang  1 , Arlene F Locke  1 , Andrea Carolina Alvarez  1 , Maria L Cabarong  1 , Lek Ching Liv  1 , Belen Garcia P Alfaro  1 , David W Gjertson  1 , Elaine F Reed  1
Affiliations

Advancing precision in histocompatibility and immunogenetics: a comprehensive review of the UCLA exchange program

Qiuheng Zhang et al. Front Genet. .

Abstract

Precise typing of human leukocyte antigens (HLA) is crucial for clinical hematopoietic stem cell and solid organ transplantations, transfusion medicine, HLA-related disease association, and drug hypersensitivity analysis. The UCLA Cell Exchange program has played a vital role in providing educational and proficiency testing surveys to HLA laboratories worldwide for the past 5 decades. This article highlights the significant contribution of the UCLA Cell and DNA Exchange Programs in advancing HLA antibody testing, genotyping, crossmatches, and, more recently, virtual crossmatches. Additionally, we discuss future directions of the UCLA Cell Exchange program to support histocompatibility testing to adapt to the fast-evolving field of immunotherapy, tolerance and xenotransplantation.

Keywords: HLA; HLA antibody; KIR; MICA; physical crossmatch; virtual crossmatch.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
The UCLA cell Exchange Program. The UCLA cell Exchange Program was first established in 1974 followed by serum exchange in 1981. In 1987, HLA class II typing was initiated with first shipment of two lymphoblastoid cell lines. By 1990, DNA typing, including restriction fragment length polymorphism (RFLP), PCR-sequence-specific primer (PCR-SSP) and PCR-sequence-specific oligonucleotide probe (PCR-SSOP) was implemented in the HLA DNA exchange survey, which became the first graded proficiency test service offered by the UCLA Exchange program in 1993. Subsequently, PCR-sequencing-based typing (PCR-SBT) was added 1996. In 2016, the exchange program embraced cutting-edge methodologies for the next-generation sequencing (NGS) HLA tying.
FIGURE 2
FIGURE 2
UCLA Virtual Crossmatch Exchange Survey. UCLA VXM Exchange consist of two Phases. In Phase I, two serum samples and three virtual donors are provided to the laboratories to perform SAB tests and 6 VXM. In Phase II, four recipient serum samples and lymphocytes from two donors are provided to perform SAB tests and 8 FXM. In Phase II, one recipient-donor pair will be selected from the VXM of Phase I, therefore a direct comparison between FXM and VXM can be achieved. VXM, virtual crossmatch; SAB, single antigen bead test; FXM, flow cytometry crossmatch; MFI, median fluorescence intensity.
See this image and copyright information in PMC

References

    1. Bahram S., Bresnahan M., Geraghty D. E., Spies T. (1994). A second lineage of mammalian major histocompatibility complex class I genes. Proc. Natl. Acad. Sci. U. S. A. 91 (14), 6259–6263. 10.1073/pnas.91.14.6259 - DOI - PMC - PubMed
    1. Bosanquet J. P., Witt C. A., Bemiss B. C., Byers D. E., Yusen R. D., Patterson A. G., et al. (2015). The impact of pre-transplant allosensitization on outcomes after lung transplantation. J. Heart Lung Transpl. 34 (11), 1415–1422. 10.1016/j.healun.2015.06.003 - DOI - PMC - PubMed
    1. Butler C. L., Hickey M. J., Jiang N., Zheng Y., Gjertson D., Zhang Q., et al. (2020). Discovery of non-HLA antibodies associated with cardiac allograft rejection and development and validation of a non-HLA antigen multiplex panel: from bench to bedside. Am. J. Transpl. 20 (10), 2768–2780. 10.1111/ajt.15863 - DOI - PMC - PubMed
    1. Cano P., Klitz W., Mack S. J., Maiers M., Marsh S. G. E., Noreen H., et al. (2007). Common and well-documented HLA alleles: report of the Ad-Hoc committee of the american society for histocompatiblity and immunogenetics. Hum. Immunol. 68 (5), 392–417. 10.1016/j.humimm.2007.01.014 - DOI - PubMed
    1. Carapito R., Aouadi I., Verniquet M., Untrau M., Pichot A., Beaudrey T., et al. (2022). The MHC class I MICA gene is a histocompatibility antigen in kidney transplantation. Nat. Med. 28 (5), 989–998. 10.1038/s41591-022-01725-2 - DOI - PMC - PubMed