doi: 10.1007/BF00508451.
Consecutive demonstration of catecholamines and dopamine-beta-hydroxylase within the same specimen
- PMID: 383661
- DOI: 10.1007/BF00508451
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Consecutive demonstration of catecholamines and dopamine-beta-hydroxylase within the same specimen
Histochemistry.
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Abstract
A combined method was developed for characterization and differentiation of catecholamines in neuron populations containing more than one catecholamine, e.g. dopamine and norepinephrine. Its application to small intensely fluorescent (SIF-) cell clusters in sympathetic ganglia allows the successive demonstration of glyoxylic acid-induced catecholamine fluorescence and dopamine-beta-hydroxylase by indirect immunofluorescence within the same tissue section. Applying this technique to an example, two types of SIF-cells were demonstrated in the guinea pig superior cervical ganglion.
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