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. 2024 Oct;69(5):1043-1052.
doi: 10.1007/s12223-024-01148-1. Epub 2024 Feb 17.

Characterization of virulence factors and antimicrobial resistance in Staphylococcus spp. isolated from clinical samples

Natalia Pedretti  1 , Ramona Iseppi  2 , Carla Condò  2 , Shakira Ghazanfar  3 , Patrizia Messi  2 , Alessandro Di Cerbo  4 , Carla Sabia  5
Affiliations

Characterization of virulence factors and antimicrobial resistance in Staphylococcus spp. isolated from clinical samples

Natalia Pedretti et al. Folia Microbiol (Praha). 2024 Oct.

Abstract

The virulence factors, antibiotic resistance patterns, and the associated genetic elements have been investigated in Staphylococcus species. A total of 100 strains has been isolated from clinical samples in the Microbiology Laboratory of Hesperia Hospital, Modena, Italy, and identified as Staphylococcus aureus (65), Staphylococcus epidermidis (24), Staphylococcus hominis (3), Staphylococcus saprophyticus (3), and Staphylococcus warneri (5). All the strains were analyzed to determine phenotypic and genotypic characters, notably the virulence factors, the antibiotics susceptibility, and the genetic determinants. The highest percentage of resistance in Staphylococcus spp. was found for erythromycin and benzylpenicillin (87% and 85%, respectively). All S. aureus, two S. epidermidis (8.3%), and one S. saprophyticus (33.3%) strains were resistant to oxacillin. The methicillin resistance gene (mecA) was detected by polymerase chain reaction (PCR) amplification in 65 S. aureus strains and in 3 coagulase-negative staphylococci (CoNS) (8.6%). With regard to the virulence characteristics, all the S. aureus were positive to all virulence tests, except for slime test. Among the CoNS isolates, 19 (79.1%) S. epidermidis and one (33.3%) S. saprophyticus strains resulted positive for the slime test only. The results obtained are useful for a more in-depth understanding of the function and contribution of S. aureus and CoNS antibiotic resistance and virulence factors to staphylococcal infections. In particular, the production of slime is very important for CoNS, a virulence factor frequently found in infections caused by these strains. Further investigations on the genetic relatedness among strains of different sources will be useful for epidemiological and monitoring purposes and will enable us to develop new strategies to counteract the diffusion of methicillin-resistant S. aureus (MRSA) and CoNS strains not only in clinical field, but also in other related environments.

Keywords: Staphylococcus spp.; Antibiotic resistance; CoNS; MRSA; Virulence factors.

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