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Comparative Study
. 2024 Jun;35(4):889-902.
doi: 10.1002/pca.3335. Epub 2024 Feb 18.

Microwave and ultrasound extraction of antioxidant phenolic compounds from Lantana camara Linn. leaves: Optimization, comparative study, and FT-Orbitrap MS analysis

Raphael Amancio de Jesus  1 , Wenes Ramos da Silva  1 , Alberto Wisniewski Jr  1 , Luís Fernando de Andrade Nascimento  2 , Arie Fitzgerald Blank  2 , Daniel Alves de Souza  3 , Elma Regina Silva de Andrade Wartha  3 , Paulo Cesar de Lima Nogueira  1 , Valéria Regina de Souza Moraes  1
Affiliations
Comparative Study

Microwave and ultrasound extraction of antioxidant phenolic compounds from Lantana camara Linn. leaves: Optimization, comparative study, and FT-Orbitrap MS analysis

Raphael Amancio de Jesus et al. Phytochem Anal. 2024 Jun.

Abstract

Introduction: The species Lantana camara is used in folk medicine. The biological activities of this medicinal plant are attributable to the presence of various derivatives of triterpenoids and phenolic compounds present in its preparations, indicating excellent economic potential.

Objective: In this study, the operational conditions of ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) were optimized using Box-Behnken design to improve the total phenolic content (TPC) recovered in hydroethanolic extracts of L. camara leaves.

Material and methods: The TPC, total flavonoid content (TFC), and antioxidant activities of the hydroalcoholic extracts of L. camara, prepared by UAE and MAE under the optimized extraction conditions, were compared with those of the extracts obtained by conventional extraction methods.

Results: Under the optimal conditions, the extracts obtained by UAE (35% ethanol, 25 min, and a solvent-to-solid ratio of 60:1 mL/g) and by MAE (53% ethanol, 15 min, and 300 W) provided high yields of 32.50% and 38.61% and TPC values of 102.89 and 109.83 mg GAE/g DW, respectively. The MAE extract showed the best results with respect to TPC, TFC, and antioxidant activities, followed by extracts obtained by UAE, Soxhlet extraction, decoction, maceration, and infusion, in that order.

Conclusion: The results obtained indicate that L. camara may be used as an important source of antioxidant phenolic compounds to obtain products with high biological and economic potential, especially when the extraction process is performed under appropriate conditions using MAE and/or UAE, employing environmentally friendly solvents such as water and ethanol.

Keywords: Verbenaceae; antioxidant activities; flavonoids; phenylpropanoids.

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References

REFERENCES

    1. Nascimento PES, Prado VMJ, Jesus RA, et al. Characterization of flavonoids profiles in polar extracts from Croton grewioides Baill. using ultra‐high resolution mass spectrometry. J Braz Chem Soc. 2023;34:937‐948. doi:10.21577/0103‐5053.20230004
    1. Georgiev M, Alipieva K, Orhan I, Abrashev R, Denev P, Angelova M. Antioxidant and cholinesterases inhibitory activities of Verbascum xanthophoeniceum Griseb. and its phenylethanoid glycosides. Food Chem. 2011;128(1):100‐105. doi:10.1016/j.foodchem.2011.02.083
    1. Veličković V, Đurović S, Radojković M, et al. Application of conventional and non‐conventional extraction approaches for extraction of Erica carnea L.: chemical profile and biological activity of obtained extracts. J Supercrit Fluids. 2017;128:331‐337. doi:10.1016/j.supflu.2017.03.023
    1. García‐Pérez E, Noratto GD, García‐Lara S, Gutiérrez‐Uribe JA, Mertens‐Talcott SU. Micropropagation effect on the anti‐carcinogenic activitiy of polyphenolics from Mexican oregano (Poliomintha glabrescens Gray) in human colon cancer cells HT‐29. Plant Foods Hum Nutr. 2013;68(2):155‐162. doi:10.1007/s11130‐013‐0344‐2
    1. Gutiérrez‐Grijalva EP, Picos‐Salas MA, Leyva‐López N, Criollo‐Mendoza MS, Vazquez‐Olivo G, Heredia JB. Flavonoids and phenolic acids from oregano: occurrence, biological activity and health benefits. Plan Theory. 2018;7(1):2‐23. doi:10.3390/plants7010002

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