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. 2024 May;341(4):421-430.
doi: 10.1002/jez.2794. Epub 2024 Feb 18.

Testosterone is closely related to Leydig cell activity, environmental factors, and androgen receptor distribution in adult male lizards of Liolaemus cuyanus (Reptilia: Liolaemidae) during the reproductive cycle

Andrea Anabella Barauna  1 , María Inés Conte  1 , Jorge Leandro Leporati  2 , Lorena Beatriz Quiroga  3 , Eduardo Alfredo Sanabria  3   4 , Miguel Walter Fornés  1
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Testosterone is closely related to Leydig cell activity, environmental factors, and androgen receptor distribution in adult male lizards of Liolaemus cuyanus (Reptilia: Liolaemidae) during the reproductive cycle

Andrea Anabella Barauna et al. J Exp Zool A Ecol Integr Physiol. 2024 May.

Abstract

Testosterone, the primary sex hormone in male lizards, is closely linked to Leydig cell activity (the cells where steroidogenesis occurs) throughout the reproductive cycle, but testosterone action is related to androgen receptors (ARs) distribution in the seminiferous epithelium. In temperate zones, environmental factors detected through the hypothalamic-pituitary-gonadal axis, downregulate plasma testosterone, resulting in a seasonal reproductive cycle. The aim of this work is to study plasma testosterone in adult male lizards of Liolaemus cuyanus, an oviparous species, throughout its reproductive cycle and its relationship with Leydig cell histology, TotalLeydigCell/ActiveLeydigCell (TLC/ALC) ratio, environmental factors (temperature, relative humidity and solar irradiation) and ARs distribution in seminiferous epithelium. Specimens (N = 27) were captured (October to March) in a semi-arid zone (Valle de Matagusanos, San Juan, Argentina) and grouped into three relevant reproductive periods: pre-reproductive (PrR), reproductive (R), and post-reproductive (PsR). Significant differences in plasma testosterone were found among these periods, highest during R than in PsR. A significant positive correlation between plasma testosterone and TLC/ALC ratio was also observed. Plasma testosterone has a significant positive correlation only with solar irradiation, but not with the other variables. In PrR and R, ARs distribution was cytoplasmic and nuclear, shifting to only cytoplasmic in PsR. These results highlight the close correspondence between plasma testosterone, Leydig cell histology and activity, environmental factors, and ARs distribution, resulting in a synchronization that allows males of L. cuyanus to coordinate their reproductive cycle with the most favorable environmental conditions, probably for mating and birth of offspring.

Keywords: histology; liolaemid; relative humidity; reproduction; solar irradiation; temperate zones; temperature.

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References

REFERENCES

    1. Abdala, C. S., Acosta, J. L., Acosta, J. C., Alvarez, B. B., Arias, F., & Ávila, L. J. (2012). Categorización del estado de conservación de de las lagartijas y anfisbenas de la República Argentina. In A. Giraudo (Ed.), Categorización del estado de conservación de las lagartijas y anfisbenas de la República Argentina (pp. 215–302). Cuadernos de Herpetología.
    1. Abu‐Zinadah, O. A. (2008). Variation in testicular histology of the spiny tailed lizard Uromastyx aegyptius microlepis during hibernation and active periods. Pakistan Journal of Biological Sciences, 11(12), 1615–1619.
    1. Aldridge, R. D., Siegel, D. S., Goldberg, S. R., & Pyron, R. A. (2020). Seasonal timing of spermatogenesis and mating in squamates: A reinterpretation. Copeia, 108(2), 231–264.
    1. Angelini, F., & Botte, V. (1992). Spermatogenesis in reptiles, dynamic and regulatory aspect. Sex Origin and Evolution, 6, 211–230.
    1. Barauna, A. A., Quiroga, L. B., Monclús, M. Á., Sanabria, E. A., & Fornés, M. W. (2023). Seasonal gonadal changes in adult male lizards of Liolaemus cuyanus (Iguania: Liolaemidae) from the Monte desert, Argentina. Theriogenology Wild, 2, 100019.

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