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. 1979 May;9(5):575-8.
doi: 10.1128/jcm.9.5.575-578.1979.

Detection of Legionella pneumonophila antigen in urine by enzyme-linked immunospecific assay

B P Berdal, C E Farshy, J C Feeley

Detection of Legionella pneumonophila antigen in urine by enzyme-linked immunospecific assay

B P Berdal et al. J Clin Microbiol. 1979 May.

Abstract

An enzyme-linked immunospecific assay "sandwich" technique was developed for detecting soluble antigen from the Legionnaires disease bacterium (Legionella pneumophila). With this technique, antigen was detected in urine specimens from guinea pigs inoculated intraperitoneally with heat-killed Legionnaires disease bacteria and in urine specimens from three of four patients who attended the American Legion Convention in Philadelphia in 1976. Urine from a fifth pneumonia patient who attended the Eucharistic Congress (but who was a dubious seroconverter) was negative. Presumably, the test could also be used for detecting antigen in sputum or respiratory aspirates, but this has not been tried to date.

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References

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    1. Lancet. 1977 Aug 6;2(8032):263-7 - PubMed
    1. J Clin Microbiol. 1979 Jan;9(1):103-7 - PubMed
    1. J Clin Microbiol. 1978 Sep;8(3):329-38 - PubMed
    1. J Clin Microbiol. 1978 Apr;7(4):327-31 - PubMed

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