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. 2024 Feb 5:15:1328632.
doi: 10.3389/fphar.2024.1328632. eCollection 2024.

Jiawei Kongsheng Zhenzhong Pill: marker compounds, absorption into the serum (rat), and Q-markers identified by UPLC-Q-TOF-MS/MS

Qiaolan Wu #  1 , Chunxue Ou #  1 , Jiayun Wang  1 , Xiaolin Wu  1 , Zu Gao  1 , Yue Zhao  2 , Guangying Lu  1   3 , Zhichun Wu  1   3 , Huayun Yu  1   3
Affiliations

Jiawei Kongsheng Zhenzhong Pill: marker compounds, absorption into the serum (rat), and Q-markers identified by UPLC-Q-TOF-MS/MS

Qiaolan Wu et al. Front Pharmacol. .

Abstract

Background: The Jiawei Kongsheng Zhenzhong pill (JKZP), a Chinese herbal prescription comprised of eight Chinese crude drugs, has been historically employed to treat neurological and psychological disorders. Nevertheless, the ambiguous material basis severely hindered its progress and application. Purpose: The current study aimed to establish a rapid analytical method for identifying the chemical components of the JKZP aqueous extract and the components absorbed into the rat serum to investigate the quality markers (Q-markers) responsible for the neuroprotective effects of JKZP. Methods: The qualitative detection of the chemical components, prototype components, and metabolites of the aqueous extracts of JKZP, as well as the serum samples of rats that were administered the drug, was performed using the ultra-performance liquid chromatography- quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) technology. This analysis combined information from literature reports and database comparisons. Moreover, the study was conducted to anticipate the potential Q-markers for the neuroprotective effects of JKZP based on the "five principles" of Q-marker determination. Results: A total of 67 compounds and 111 serum components (comprising 33 prototypes and 78 metabolites) were detected and identified. Combining the principles of quality transmission and traceability, compound compatibility environment, component specificity, effectiveness, and measurability, the study predicted that five key compounds, namely, senkyunolide H, danshensu, echinacoside, loganin, and 3,6'-disinapoyl sucrose, may serve as potential pharmacological bases for the neuroprotective effects of JKZP. Conclusion: To summarize, the UPLC-Q-TOF-MS/MS technique can be employed to rapidly and accurately identify compounds in JKZP. Five active compounds have been predicted to be the Q-markers for the neuroprotective effects of JKZP. This discovery serves as a reference for improving quality, advancing further research and development, and utilizing Chinese herbal prescriptions.

Keywords: Chinese herbal prescription; Jiawei Kongsheng Zhenzhong Pill; compounds; neuroprotective effect; pharmacodynamic material basis; quality marker; ultra-performance liquid chromatography quadrupole time-of-flight mass tandem spectrometry.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Total ion flow diagram of the JKZP aqueous extract by UPLC-HRMS: (A) negative ion modes; (B) positive ion modes.
FIGURE 2
FIGURE 2
Components in the JKZP aqueous extract, prototypical components, and metabolites in JKZP-containing serum were intersected.
FIGURE 3
FIGURE 3
Steps in predicting Q-markers of JKZP.
FIGURE 4
FIGURE 4
(A–E) Chemical structures of JKZP Q-markers. (A) Senkyunolide H; (B) danshensu; (C) echinacoside; (D) loganic acid; (E) 3,6′-disinapoyl sucrose.
See this image and copyright information in PMC

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