Plant biotechnology research with single-cell transcriptome: recent advancements and prospects

doi:10.1007/s00299-024-03168-0

Review

. 2024 Feb 21;43(3):75.

doi: 10.1007/s00299-024-03168-0.

Plant biotechnology research with single-cell transcriptome: recent advancements and prospects

Muhammad Ali^#^{1

2}, Tianxia Yang^#^{1

3}, Hai He¹, Yu Zhang⁴

Affiliations

¹ School of Agriculture, Sun Yat-Sen University, Shenzhen, 518107, China.
² Peking University-Institute of Advanced Agricultural Sciences, Weifang, China.
³ State Key Laboratory of Maize Bio-breeding, National Maize Improvement Center, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, China.
⁴ School of Agriculture, Sun Yat-Sen University, Shenzhen, 518107, China. zhangy2526@mail.sysu.edu.cn.

^# Contributed equally.

PMID: 38381195
DOI: 10.1007/s00299-024-03168-0

Review

Plant biotechnology research with single-cell transcriptome: recent advancements and prospects

Muhammad Ali et al. Plant Cell Rep. 2024.

. 2024 Feb 21;43(3):75.

doi: 10.1007/s00299-024-03168-0.

Authors

Muhammad Ali^#^{1

2}, Tianxia Yang^#^{1

3}, Hai He¹, Yu Zhang⁴

Affiliations

¹ School of Agriculture, Sun Yat-Sen University, Shenzhen, 518107, China.
² Peking University-Institute of Advanced Agricultural Sciences, Weifang, China.
³ State Key Laboratory of Maize Bio-breeding, National Maize Improvement Center, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, China.
⁴ School of Agriculture, Sun Yat-Sen University, Shenzhen, 518107, China. zhangy2526@mail.sysu.edu.cn.

^# Contributed equally.

PMID: 38381195
DOI: 10.1007/s00299-024-03168-0

Abstract

Single-cell transcriptomic techniques have emerged as powerful tools in plant biology, offering high-resolution insights into gene expression at the individual cell level. This review highlights the rapid expansion of single-cell technologies in plants, their potential in understanding plant development, and their role in advancing plant biotechnology research. Single-cell techniques have emerged as powerful tools to enhance our understanding of biological systems, providing high-resolution transcriptomic analysis at the single-cell level. In plant biology, the adoption of single-cell transcriptomics has seen rapid expansion of available technologies and applications. This review article focuses on the latest advancements in the field of single-cell transcriptomic in plants and discusses the potential role of these approaches in plant development and expediting plant biotechnology research in the near future. Furthermore, inherent challenges and limitations of single-cell technology are critically examined to overcome them and enhance our knowledge and understanding.

Keywords: Plant biotechnology; Plant development; Plant transcriptome; Single-cell technology; Spatial transcriptome; scRNA-seq.

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References

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[1] Abdelaal T, Michielsen L, Cats D et al (2019) A comparison of automatic cell identification methods for single-cell RNA sequencing data. Genome Biol. https://doi.org/10.1186/s13059-019-1795-z - DOI - PubMed - PMC

[2] Abdelaal T, Michielsen L, Cats D et al (2019) A comparison of automatic cell identification methods for single-cell RNA sequencing data. Genome Biol. https://doi.org/10.1186/s13059-019-1795-z - DOI - PubMed - PMC

[3] Adrian J, Chang J, Ballenger CE et al (2015) Transcriptome dynamics of the stomatal lineage: birth, amplification, and termination of a self-renewing population. Dev Cell 33:107–118. https://doi.org/10.1016/j.devcel.2015.01.025 - DOI - PubMed - PMC

[4] Adrian J, Chang J, Ballenger CE et al (2015) Transcriptome dynamics of the stomatal lineage: birth, amplification, and termination of a self-renewing population. Dev Cell 33:107–118. https://doi.org/10.1016/j.devcel.2015.01.025 - DOI - PubMed - PMC

[5] Aldridge S, Teichmann SA (2020) Single cell transcriptomics comes of age. Nat Commun 11:4307. https://doi.org/10.1038/s41467-020-18158-5 - DOI - PubMed - PMC

[6] Aldridge S, Teichmann SA (2020) Single cell transcriptomics comes of age. Nat Commun 11:4307. https://doi.org/10.1038/s41467-020-18158-5 - DOI - PubMed - PMC

[7] Asp M, Giacomello S, Larsson L et al (2019) A spatiotemporal organ-wide gene expression and cell atlas of the developing human heart. Cell 179:1647-1660.e19. https://doi.org/10.1016/j.cell.2019.11.025 - DOI - PubMed

[8] Asp M, Giacomello S, Larsson L et al (2019) A spatiotemporal organ-wide gene expression and cell atlas of the developing human heart. Cell 179:1647-1660.e19. https://doi.org/10.1016/j.cell.2019.11.025 - DOI - PubMed

[9] Bakken TE, Hodge RD, Miller JA et al (2018) Single-nucleus and single-cell transcriptomes compared in matched cortical cell types. PLoS ONE 13:e0209648. https://doi.org/10.1371/journal.pone.0209648 - DOI - PubMed - PMC

[10] Bakken TE, Hodge RD, Miller JA et al (2018) Single-nucleus and single-cell transcriptomes compared in matched cortical cell types. PLoS ONE 13:e0209648. https://doi.org/10.1371/journal.pone.0209648 - DOI - PubMed - PMC

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Plant biotechnology research with single-cell transcriptome: recent advancements and prospects

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Plant biotechnology research with single-cell transcriptome: recent advancements and prospects

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