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. 2024:2765:209-226.
doi: 10.1007/978-1-0716-3678-7_12.

Directed Circularization of a Short RNA

Cathrin E Hansen  1 , Danilo Springstubbe  1 , Sabine Müller  1 , Sonja Petkovic  2
Affiliations

Directed Circularization of a Short RNA

Cathrin E Hansen et al. Methods Mol Biol. 2024.

Abstract

Basic research and functional analyses of circular RNA (circRNA) have been limited by challenges in circRNA formation of desired length and sequence in adequate yields. Nowadays, circular RNA can be obtained using enzymatic, "ribozymatic," or modulated splice events. However, there are few records for the directed circularization of RNA. Here, we present a proof of principle for an affordable and efficient RNA-based system for the controlled synthesis of circRNA with a physiological 3',5'-phosphodiester conjunction. The engineered hairpin ribozyme variant circular ribozyme 3 (CRZ-3) performs self-cleavage poorly. We designed an activator-polyamine complex to complete cleavage as a prerequisite for subsequent circularization. The developed protocol allows synthesizing circRNA without external enzymatic assistance and adds a controllable way of circularization to the existing methods.

Keywords: Activator; Bioengineering; Catalysis; Circular; Circularization; RNA; Ribozyme; Spermine.

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