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. 2023 Jan 18;68(1):27-32.
doi: 10.17221/57/2022-VETMED. eCollection 2023 Jan.

Antimicrobial activities of nano-emulsion of virgin coconut oil

Desy Cahya Widianingrum  1 , Himmatul Khasanah  1 , Listya Purnamasari  1 , Melinda Erdya Krismaputri  1 , Seong Gu Hwang  2
Affiliations

Antimicrobial activities of nano-emulsion of virgin coconut oil

Desy Cahya Widianingrum et al. Vet Med (Praha). .

Abstract

This study aimed to determine the nano-emulsion of virgin coconut oil (n-VCO) formula that can produce the best size and zone inhibition of antimicrobial activity. The VCO was formulated with the different percentages of Tween 80 (P1: 24%, P2: 25%, P3: 26%) and sorbitol (P1: 36%, P2: 35%, P3: 34%). The particle size of the n-VCO emulsion was observed under transmission electron microscopy (TEM). The antimicrobial activity test of the n-VCO was determined by a challenge test using Salmonella Typhi (S. Typhi), Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli) bacteria. The data were analysed by a one-way ANOVA (P < 0.05). The significant data were furthermore tested by Duncan's multiple ranges (SPSS v26.0). This study showed that the P3 formulation (26% Tween 80 and 34% sorbitol) produced the best n-VCO among all the treatments showing a particle size of 5-100 nm. Formulas P1 and P2 produced particle sizes of about 500-1 000 nm. The antimicrobial test showed that the P3 formula had a strong inhibitory effect on S. Typhi (7.442 ± 0.52 mm), S. aureus (8.380 ± 0.49 mm), and E. coli (6.490 ± 0.82 mm). This study concluded that the formula of the detergent strongly influences the particle size of the n-VCO. The n-VCO has enormous potential to be used as an alternative antimicrobial.

Keywords: alternative antimicrobial; nano-emulsification formula; natural resources.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1. Particle size of the nano-emulsion of VCO photographed using transmission electron microscopy (TEM)
P1 = particle size 500–1 000 nm; P2 = particle size 500–1 000 nm; P3 = particle size 5–100 nm; VCO = virgin coconut oil
See this image and copyright information in PMC

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