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. 2024 Oct;196(10):6608-6623.
doi: 10.1007/s12010-024-04885-8. Epub 2024 Feb 22.

Preparation of Yeast Extract from Brewer's Yeast Waste and Its Potential Application as a Medium Constituent

Feng Gao  1 , Qingfei Li  1 , Wei Wei  1 , Yutao Wang  1 , Wancheng Song  1 , Xurui Yang  1 , Huai Ji  1 , Jia Zhou  1 , Ya Xin  1 , Zhongbiao Tan  1 , Jianjun Pei  2 , Hao Shi  3
Affiliations

Preparation of Yeast Extract from Brewer's Yeast Waste and Its Potential Application as a Medium Constituent

Feng Gao et al. Appl Biochem Biotechnol. 2024 Oct.

Abstract

Yeast extract serves as a source of nutritional components essential for human dietary requirements, feed formulations, and the vital growth factors and nutrients necessary for microorganisms. However, the production cost of yeast extract using cultivated active dry yeast is relatively high. This study aims to utilize the autolysis of discarded yeast post beer brewing to produce yeast extract. The concentration, temperature, pH, and time conditions are systematically optimized. It reveals that the yield of amino nitrogen and solids in the extract was increased by 3.3% and 20.9% under the optimized conditions (1.2% wall-breaking enzyme, 1% yeast extract enzyme, and a hydrolysis time of 24 h) than that of the documented 4.03% and 69.05%. Additionally, a comparative analysis with commercially available yeast powder demonstrates that the yeast extract derived from this study adequately fulfills the nutritional requirements for microbial growth. Hence, the utilization of discarded beer yeast presents an opportunity for the valuable reclamation of waste yeast, showcasing promising potential applications.

Keywords: Autolysis; Brewer’s yeast; Medium; Yeast extract.

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Conflict of interest statement

Declarations Ethics Approval Not applicable. Consent to Participate Not applicable. Consent for Publication Not applicable. Competing Interests The authors declare no competing interests.

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