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. 2024 Jan-Mar;15(1):37-42.
doi: 10.4103/JAPTR.JAPTR_267_23. Epub 2024 Jan 15.

Characteristics of Lactobacillus casei probiotic microparticles in L-type methacrylic acid copolymer matrix

Sugiyartono  1 , Widji Soeratri  1 , Arini Permatasari  1 , Ayun Dewi Rahayu  1 , Dwi Setyawan  1 , Dewi Isadiartuti  1
Affiliations

Characteristics of Lactobacillus casei probiotic microparticles in L-type methacrylic acid copolymer matrix

Sugiyartono et al. J Adv Pharm Technol Res. 2024 Jan-Mar.

Abstract

Lactobacillus casei (LC) is a type of lactic acid bacterium that is known for its beneficial probiotic properties. However, it is not typically found in the human intestine because it lacks acid resistance. LC thrives in an optimal pH environment of 6.8 and can be initiated in a more acidic environment at a pH of 3.5. This study purposed to compare the effect of L-type methacrylic acid copolymer (MAC) as a matrix (0.50%, 0.75%, and 1.00%) on the physical characteristics of LC probiotic microparticles made by the spray drying process. Probiotic microparticles were also made from a dry suspension of LC FNCC 0090 bacteria and dispersed in a solution of L-type MAC. The results showed that a rise in matrix content by 1.00% increased particle size (4.47 ± 0.19 µm) and reduced moisture content (7.45 ± 0.11%). The analysis of microparticle morphology also indicated a positive correlation between the level of L-type MAC and the production of smooth, nonporous, and almost spherical shapes. In addition, it was observed that encapsulation efficiency (92.46 ± 0.17%) and protection against stomach acid (98.17% ±1.17%) increased with the level of the matrix.

Keywords: L-type methacrylic acid copolymer; Lactobacillus casei; microencapsulation; probiotic; spray drying.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Graph of the relationship between growth time vs. pH media and log TPC. TPC: Total plate count
Figure 2
Figure 2
Photograph of Lactobacillus casei probiotic microparticles using L-type methacrylic acid copolymer of FI (a), FII (b), and FIII (c) taken with scanning electron microscopy
Figure 3
Figure 3
Comparison of the Fourier transform infrared spectroscopy spectra of L-type methacrylic acid copolymer and microparticles FI, FII, and FIII
See this image and copyright information in PMC

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