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. 2024 Feb 17;14(2):106.
doi: 10.3390/bios14020106.

Enhanced Nanozymatic Activity on Rough Surfaces for H2O2 and Tetracycline Detection

Tawfiq Alsulami  1 , Abdulhakeem Alzahrani  1
Affiliations

Enhanced Nanozymatic Activity on Rough Surfaces for H2O2 and Tetracycline Detection

Tawfiq Alsulami et al. Biosensors (Basel). .

Abstract

The needless use of tetracyclines (TCs) in foodstuffs is a huge health concern in low- and middle-income and Arab countries. Herein, a sensitive and faster monitoring system for H2O2 and TCs is proposed, utilizing the large surface-to-volume ratio of a non-spherical gold nanoparticle/black phosphorus nanocomposite (BP-nsAu NPs) for the first time. BP-nsAu NPs were synthesized through a single-step method that presented nanozymatic activity through 3,3',5,5'-Tetramethylbenzidine (TMB) oxidation while H2O2 was present and obeyed the Michaelis-Menten equation. The nanozymatic activity of the BP-nsAu NPs was enhanced 12-fold and their detection time was decreased 83-fold compared to conventional nanozymatic reactions. The proposed method enabled us to quantify H2O2 with a limit of detection (LOD) value of 60 nM. Moreover, target-specific aptamer-conjugated BP-nsAu NPs helped us detect TCs with an LOD value of 90 nM. The present strategy provides a proficient route for low-level TC monitoring in real samples.

Keywords: H2O2; black phosphorus; nanozyme; spiky gold nanoparticle; tetracyclines.

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Conflict of interest statement

The authors declare no competing financial interests.

Figures

Scheme 1
Scheme 1
Schematic presentation of the proposed sensing strategy.
Figure 1
Figure 1
Characterization of BP-Au NPs: (A) absorbance spectra of composite; (B) far-view TEM image of BP-sAu NPs; (C) close-view TEM image of BP-sAu NPs; and (D) TEM image of BP-Au NPs.
Figure 2
Figure 2
Optimization of (A) pH; (B) reaction time; (C) concentration of H2O2; and (D) concentration of TMBZ.
Figure 3
Figure 3
(A) Comparison study of the nanozymatic activity of pristine BP (blue line); BP-sAuNPs (red line), and BP-nsAu NPs (black line); (B) TA test result (red: in the presence of BP-nsAu, TA, and H2O2 and black: BP-nsAu NPs and TA) and (C) DPPH results of BP-nsAu NPs and SCN-modified BP-nsAu NPs.
Figure 4
Figure 4
(A) Nanozymatic detection of H2O2 using a conventional method; (B) absorbance (at 660 nm) using electrochemical potential and the conventional method and (C) nanozymatic detection of H2O2 using electrochemical potential.
Figure 5
Figure 5
(A) Nanozymatic detection of TCs under electrochemical potential; (B) selectivity of TC detection.
See this image and copyright information in PMC

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