Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Feb 17;14(2):106.
doi: 10.3390/bios14020106.

Enhanced Nanozymatic Activity on Rough Surfaces for H2O2 and Tetracycline Detection

Tawfiq Alsulami  1 , Abdulhakeem Alzahrani  1
Affiliations

Enhanced Nanozymatic Activity on Rough Surfaces for H2O2 and Tetracycline Detection

Tawfiq Alsulami et al. Biosensors (Basel). .

Abstract

The needless use of tetracyclines (TCs) in foodstuffs is a huge health concern in low- and middle-income and Arab countries. Herein, a sensitive and faster monitoring system for H2O2 and TCs is proposed, utilizing the large surface-to-volume ratio of a non-spherical gold nanoparticle/black phosphorus nanocomposite (BP-nsAu NPs) for the first time. BP-nsAu NPs were synthesized through a single-step method that presented nanozymatic activity through 3,3',5,5'-Tetramethylbenzidine (TMB) oxidation while H2O2 was present and obeyed the Michaelis-Menten equation. The nanozymatic activity of the BP-nsAu NPs was enhanced 12-fold and their detection time was decreased 83-fold compared to conventional nanozymatic reactions. The proposed method enabled us to quantify H2O2 with a limit of detection (LOD) value of 60 nM. Moreover, target-specific aptamer-conjugated BP-nsAu NPs helped us detect TCs with an LOD value of 90 nM. The present strategy provides a proficient route for low-level TC monitoring in real samples.

Keywords: H2O2; black phosphorus; nanozyme; spiky gold nanoparticle; tetracyclines.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing financial interests.

Figures

Scheme 1
Scheme 1
Schematic presentation of the proposed sensing strategy.
Figure 1
Figure 1
Characterization of BP-Au NPs: (A) absorbance spectra of composite; (B) far-view TEM image of BP-sAu NPs; (C) close-view TEM image of BP-sAu NPs; and (D) TEM image of BP-Au NPs.
Figure 2
Figure 2
Optimization of (A) pH; (B) reaction time; (C) concentration of H2O2; and (D) concentration of TMBZ.
Figure 3
Figure 3
(A) Comparison study of the nanozymatic activity of pristine BP (blue line); BP-sAuNPs (red line), and BP-nsAu NPs (black line); (B) TA test result (red: in the presence of BP-nsAu, TA, and H2O2 and black: BP-nsAu NPs and TA) and (C) DPPH results of BP-nsAu NPs and SCN-modified BP-nsAu NPs.
Figure 4
Figure 4
(A) Nanozymatic detection of H2O2 using a conventional method; (B) absorbance (at 660 nm) using electrochemical potential and the conventional method and (C) nanozymatic detection of H2O2 using electrochemical potential.
Figure 5
Figure 5
(A) Nanozymatic detection of TCs under electrochemical potential; (B) selectivity of TC detection.
See this image and copyright information in PMC

References

    1. Ahmed S.R., Chand R., Kumar S., Mittal N., Srinivasan S., Rajabzadeh A. Recent Biosensing Advances in the Rapid Detection of Illicit Drugs. TrAC Trends Anal. Chem. 2020;131:116006. doi: 10.1016/j.trac.2020.116006. - DOI
    1. Ahmed S.R., Cardoso A.G., Kumar S., Ortega G.A., Srinivasan S., Rajabzadeh A.R. Nanozymes in Biosensing and Bioimaging. CRC Press; Boca Raton, FL, USA: 2021. pp. 115–143. Chapter 7.
    1. Ahmed S.R., Sherazee M., Srinivasan S., Rajabzadeh A.R. Nanozymatic Detection of Thiocyanate through Accelerating the Growth of Ultra-small Gold Nanoparticles/Graphene Quantum Dots Hybrids. Food Chem. 2022;379:132152. doi: 10.1016/j.foodchem.2022.132152. - DOI - PubMed
    1. Ahmed S.R., Sherazee M., Srinivasan S., Rajabzadeh A.R. Positively Charged Gold Quantum Dots: An Nanozymatic “Off-On” Sensor for Thiocyanate Detection. Foods. 2022;11:1189. doi: 10.3390/foods11091189. - DOI - PMC - PubMed
    1. Ahmed S.R., Cardoso A.G., Cobas H.V., Das P., Srinivasan S., Rajabzadeh A.R. Graphdiyne Quantum Dots for H2O2 and Dopamine Detection. ACS Appl. Nano Mater. 2023;10:8434–8443. doi: 10.1021/acsanm.3c00771. - DOI

LinkOut - more resources