Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Feb 23;103(8):e37175.
doi: 10.1097/MD.0000000000037175.

No potential causal link between HP infection and IBD: A 2way Mendelian randomization study

Affiliations

No potential causal link between HP infection and IBD: A 2way Mendelian randomization study

Kaiqi Yang et al. Medicine (Baltimore). .

Abstract

Recent epidemiological research suggests a possible negative correlation between Helicobacter pylori infection and inflammatory bowel disease (IBD). However, conflicting studies have provided unclear evidence regarding these causal relationships. Therefore, recommending specific prevention and treatment strategies for H. pylori infection and IBD is challenging. We used various antibodies (anti-H. pylori IgG, VacA, and GroEl) related to H. pylori infection as indicators. We acquired relevant genetic variants from public databases within the Genome-wide Association Studies (GWAS) dataset using IBDs tool variables from 2 different GWAS datasets. We thoroughly examined the data and screened for IVs that fulfilled these criteria. Subsequently, Bidirectional Mendelian randomization (MR) was conducted to predict the potential causality between the 2. To ensure the accuracy and robustness of our results, we conducted a series of sensitivity analyses. Based on our comprehensive MR analysis, no potential causal relationship was observed between H. pylori infection and IBD. Across various methodologies, including IVW, MR-Egger, and weighted median, our findings showed P values > .05. The only exception was observed in the reverse MR analysis using the MR-Egger method, which yielded a P value of < .05. However, because the IVW method is considered the most statistically significant method for MR, and its P value was > .05, we do not believe that a potential causal relationship exists between them. Our sensitivity analysis did not suggest significant horizontal pleiotropism. Although heterogeneity was detected in the analysis of IBD (IIBDGC source) versus H. pylori GroEL antibody levels (MR-Egger, Qp = 0.038; IVW, Qp = 0.043), the results remained reliable because we selected IVW as a random-effects model in our MR analysis method. Based on our MR research, no direct correlation was observed between H. pylori infection and IBD risk. This implies that eradicating H. pylori may not provide substantial benefits in preventing or treating regional IBD, and vice versa. Nevertheless, the use of H. pylori serological index substitution has limitations, and further research using histological diagnosis and additional MR studies is required to comprehensively assess the link between H. pylori infection and IBD.

PubMed Disclaimer

Conflict of interest statement

The authors have no funding and conflicts of interest to disclose.

Figures

Figure 1.
Figure 1.
Schematic representation of the bidirectional MR study of the causal relationship between H. pylori infection and IBD. SNPs, single-nucleotide polymorphisms. H. pylori, Helicobacter pylori.
Figure 2.
Figure 2.
Forest plot of Mendelian randomization analyses. OR, odds ratio. 95%CI, 95% confidence interval. nsnp, number of single-nucleotide polymorphisms. HP_IgG, Anti-H. pylori IgG seropositivity. HP_VacA, H. pylori VacA antibody levels. HP_GroEL, H. pylori GroEL antibody levels. IBD_Finn, GWAS data source of inflammatory bowel disease in the FinnGen database. IBD_IIBDGG, GWAS data source of inflammatory bowel disease from the International Inflammatory Bowel Disease Genetics Consortium.
Figure 3.
Figure 3.
Scatterplot of significant causal relationships between H. pylori infection-related antibody markers and IBD. IBD (Finn), GWAS data source of inflammatory bowel disease in the FinnGen database. IBD (IIBDGG), GWAS data source of inflammatory bowel disease from the International Inflammatory Bowel Disease Genetics Consortium.
Figure 4.
Figure 4.
Forest plot of reverse Mendelian randomization analyses. OR, odds ratio. 95%CI, 95% confidence interval. HP_IgG, Anti-H. pylori IgG seropositivity. HP_VacA, H. pylori VacA antibody levels. HP_GroEL, H. pylori GroEL antibody levels. IBD_Finn, GWAS data source of inflammatory bowel disease in the FinnGen database. IBD_IIBDGG, GWAS data source of inflammatory bowel disease from the International Inflammatory Bowel Disease Genetics Consortium.

Similar articles

Cited by

References

    1. Bernstein CN, Fried M, Krabshuis J, et al. . Inflammatory bowel disease: a global perspective. 2009.
    1. Kaplan GG. The global burden of IBD: from 2015 to 2025. Nat Rev Gastroenterol Hepatol. 2015;12:720–7. - PubMed
    1. Molodecky NA, Soon IS, Rabi DM, et al. . Increasing incidence and prevalence of the inflammatory bowel diseases with time, based on systematic review. Gastroenterology. 2012;142:46–54.e42; quiz e30. - PubMed
    1. Danese S, Fiocchi C. Etiopathogenesis of inflammatory bowel diseases. World J Gastroenterol. 2006;12:4807–12. - PMC - PubMed
    1. Kugathasan S, Fiocchi C. Progress in basic inflammatory bowel disease research. Semin Pediatr Surg. 2007;16:146–53. - PubMed

MeSH terms

Substances