Heterogeneity of membrane phospholipid mobility in endothelial cells depends on cell substrate
- PMID: 3839906
- DOI: 10.1038/317075a0
Heterogeneity of membrane phospholipid mobility in endothelial cells depends on cell substrate
Abstract
Cellular growth control and differentiation have been shown to be dependent on both cell-cell and cell-substrate contacts. Interactions of cells with extracellular material are critical events during embryonic development and maintenance of tissue function. Plasma membrane receptors have been described for components of the extracellular matrix such as fibronectin, laminin and various collagen types. Transmembrane signalling has been shown to be influenced by the lateral mobilities of the plasma membrane constituents. The interaction of cells with their extracellular matrix could thus have a significant effect on the mobility properties of the plasma membrane components. Here we have studied the dynamic properties of fluorescent membrane phospholipids in bovine endothelial cells using fluorescence recovery after photo-bleaching measurements. At this molecular level we find that the phospholipid lateral diffusion coefficient is dependent on the substrate upon which cells are allowed to adhere (collagen, fibronectin or a natural basement membrane) and on the topography of the cell (basal versus apical plasma membrane).
Similar articles
-
Effect of plasma membrane fluidity on serotonin transport by endothelial cells.Am J Physiol. 1987 Nov;253(5 Pt 1):C672-8. doi: 10.1152/ajpcell.1987.253.5.C672. Am J Physiol. 1987. PMID: 3688215
-
Differences in the transbilayer and lateral motions of fluorescent analogs of phosphatidylcholine and phosphatidylethanolamine in the apical plasma membrane of bovine aortic endothelial cells.Exp Cell Res. 1993 Oct;208(2):387-97. doi: 10.1006/excr.1993.1260. Exp Cell Res. 1993. PMID: 8375469
-
Quantitative contributions of cholesterol and the individual classes of phospholipids and their degree of fatty acyl (un)saturation to membrane fluidity measured by fluorescence polarization.Biochemistry. 1987 Mar 24;26(6):1746-56. doi: 10.1021/bi00380a038. Biochemistry. 1987. PMID: 3593687
-
Expression and function of laminins in the embryonic and mature vasculature.Physiol Rev. 2005 Jul;85(3):979-1000. doi: 10.1152/physrev.00014.2004. Physiol Rev. 2005. PMID: 15987800 Review.
-
Membrane fluidity of blood cells.Haematologia (Budap). 1996;27(3):109-27. Haematologia (Budap). 1996. PMID: 14653448 Review.
Cited by
-
High spatial-resolved heat manipulating membrane heterogeneity alters cellular migration and signaling.Proc Natl Acad Sci U S A. 2023 Nov 28;120(48):e2312603120. doi: 10.1073/pnas.2312603120. Epub 2023 Nov 20. Proc Natl Acad Sci U S A. 2023. PMID: 37983503 Free PMC article.
-
Lateral mobility of class I histocompatibility antigens in B lymphoblastoid cell membranes: modulation by cross-linking and effect of cell density.J Cell Biol. 1987 Sep;105(3):1147-52. doi: 10.1083/jcb.105.3.1147. J Cell Biol. 1987. PMID: 3115995 Free PMC article.
-
Tumor necrosis factor and immune interferon act in concert to slow the lateral diffusion of proteins and lipids in human endothelial cell membranes.J Cell Biol. 1988 Aug;107(2):781-9. doi: 10.1083/jcb.107.2.781. J Cell Biol. 1988. PMID: 3138247 Free PMC article.
-
Cheek cell membrane fluidity measured by fluorescence recovery after photobleaching and steady-state fluorescence anisotropy.J Membr Biol. 1994 Nov;142(2):223-8. doi: 10.1007/BF00234944. J Membr Biol. 1994. PMID: 7884814
-
Quantitative imaging of free and total intracellular calcium in cultured cells.Proc Natl Acad Sci U S A. 1989 Mar;86(6):1870-4. doi: 10.1073/pnas.86.6.1870. Proc Natl Acad Sci U S A. 1989. PMID: 2928310 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources