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. 2024 Feb 13;12(2):382.
doi: 10.3390/microorganisms12020382.

Evaluation of Autof MS2600 and MBT Smart MALDI-TOF MS Systems for Routine Identification of Clinical Bacteria and Yeasts

Elena De Carolis  1 , Vittorio Ivagnes  1 , Carlotta Magrì  1 , Benedetta Falasca  1 , Teresa Spanu  1 , Maurizio Sanguinetti  1
Affiliations

Evaluation of Autof MS2600 and MBT Smart MALDI-TOF MS Systems for Routine Identification of Clinical Bacteria and Yeasts

Elena De Carolis et al. Microorganisms. .

Abstract

The identification of microorganisms at the species level has always constituted a diagnostic challenge for clinical microbiology laboratories. The aim of the present study has been the evaluation in a real-time assay of the performance of Autobio in comparison with the Bruker mass spectrometry system for the identification of bacteria and yeasts. A total of 535 bacteria and yeast were tested in parallel with the two systems by direct smear or fast formic acid extraction for bacteria and yeasts, respectively. Discordant results were verified by 16S, ITS rRNA or specific gene sequencing. Beyond giving comparable results for bacteria with respect to the MBT smart system, Autof MS2600 mass spectrometer provided excellent accuracy for the identification of yeast species of clinical interest.

Keywords: MALDI-TOF MS; clinical microbial identification; evaluation Autof MS2600.

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Conflict of interest statement

The authors declare no conflict of interest.

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