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. 2024 Jul;22(7):1957-1959.
doi: 10.1111/pbi.14314. Epub 2024 Feb 23.

Optimizing ex vitro one-step RUBY-equipped hairy root transformation in drug- and hemp-type Cannabis

Ladan Ajdanian  1   2   3   4 , Mohsen Niazian  1   2   3   4 , Davoud Torkamaneh  1   2   3   4
Affiliations

Optimizing ex vitro one-step RUBY-equipped hairy root transformation in drug- and hemp-type Cannabis

Ladan Ajdanian et al. Plant Biotechnol J. 2024 Jul.
No abstract available

Keywords: Agrobacterium rhizogenes; cannabis; hairy root transformation; hairy root culture; transformation; transformation efficiency.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
One‐step hairy root induction and RUBY gene expression in cannabis. (a1) The emergence of red hairy roots expressing RUBY gene in cannabis (bar = 1 cm). (a2) The elongation of red hairy root expressing RUBY reporter gene (bar = 1 cm). (b) A fully developed composite cannabis plant (bar = 1 cm). (c) Mean comparison ± standard error (LSD P ≤ 0.05) with two different types of seed on percentage of explants showing hairy root induction in cannabis. (d) Mean comparison ± standard error (LSD P ≤ 0.05) with three different stains on percent of transformed roots in cannabis. Different letters denote treatments that produce statistically different means.
See this image and copyright information in PMC

References

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