Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2024 Apr;41(4):158-170.
doi: 10.1002/yea.3934. Epub 2024 Feb 25.

The rise of single-cell transcriptomics in yeast

Mariona Nadal-Ribelles  1   2 , Carme Solé  1   2 , Eulalia de Nadal  1   2 , Francesc Posas  1   2
Affiliations
Free article
Review

The rise of single-cell transcriptomics in yeast

Mariona Nadal-Ribelles et al. Yeast. 2024 Apr.
Free article

Abstract

The field of single-cell omics has transformed our understanding of biological processes and is constantly advancing both experimentally and computationally. One of the most significant developments is the ability to measure the transcriptome of individual cells by single-cell RNA-seq (scRNA-seq), which was pioneered in higher eukaryotes. While yeast has served as a powerful model organism in which to test and develop transcriptomic technologies, the implementation of scRNA-seq has been significantly delayed in this organism, mainly because of technical constraints associated with its intrinsic characteristics, namely the presence of a cell wall, a small cell size and little amounts of RNA. In this review, we examine the current technologies for scRNA-seq in yeast and highlight their strengths and weaknesses. Additionally, we explore opportunities for developing novel technologies and the potential outcomes of implementing single-cell transcriptomics and extension to other modalities. Undoubtedly, scRNA-seq will be invaluable for both basic and applied yeast research, providing unique insights into fundamental biological processes.

Keywords: methods; single‐cell transcriptomics; transcription; yeast.

PubMed Disclaimer

References

REFERENCES

    1. Ahmad, R., & Budnik, B. (2023). A review of the current state of single‐cell proteomics and future perspective. Analytical and Bioanalytical Chemistry, 415, 6889–6899. https://doi.org/10.1007/S00216-023-04759-8/TABLES/1
    1. Bertrand, E., Chartrand, P., Schaefer, M., Shenoy, S. M., Singer, R. H., & Long, R. M. (1998). Localization of ASH1 mRNA particles in living yeast. Molecular Cell, 2(4), 437–445. https://doi.org/10.1016/S1097-2765(00)80143-4
    1. Blair, J. D., Hartman, A., Zenk, F., Dalgarno, C., Treutlein, B., & Satija, R. (2023). Phospho‐seq: Integrated, multi‐modal profiling of intracellular protein dynamics in single cells. bioRxiv. https://doi.org/10.1101/2023.03.27.534442
    1. Boocock, J., Alexander, N., Tapia, L. A., Walter‐Mcneill, L., Munugala, C., Bloom, J. S., & Kruglyak, L. (2023). Single‐cell eQTL mapping in yeast reveals a tradeoff between growth and reproduction. bioRxiv. https://doi.org/10.1101/2023.12.07.570640
    1. Braselmann, E., Rathbun, C., Richards, E. M., & Palmer, A. E. (2020). Illuminating RNA biology: Tools for imaging RNA in live mammalian cells. Cell Chemical Biology, 27(8), 891–903. https://doi.org/10.1016/J.CHEMBIOL.2020.06.010

Publication types