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Review
. 2024 Feb 6;9(7):7375-7392.
doi: 10.1021/acsomega.3c08930. eCollection 2024 Feb 20.

Decellularization and Their Significance for Tissue Regeneration in the Era of 3D Bioprinting

Mrunmayi Gadre  1 , Meghana Kasturi  2 , Prachi Agarwal  1 , Kirthanashri S Vasanthan  1
Affiliations
Review

Decellularization and Their Significance for Tissue Regeneration in the Era of 3D Bioprinting

Mrunmayi Gadre et al. ACS Omega. .

Abstract

Three-dimensional bioprinting is an emerging technology that has high potential application in tissue engineering and regenerative medicine. Increasing advancement and improvement in the decellularization process have led to an increase in the demand for using a decellularized extracellular matrix (dECM) to fabricate tissue engineered products. Decellularization is the process of retaining the extracellular matrix (ECM) while the cellular components are completely removed to harvest the ECM for the regeneration of various tissues and across different sources. Post decellularization of tissues and organs, they act as natural biomaterials to provide the biochemical and structural support to establish cell communication. Selection of an effective method for decellularization is crucial, and various factors like tissue density, geometric organization, and ECM composition affect the regenerative potential which has an impact on the end product. The dECM is a versatile material which is added as an important ingredient to formulate the bioink component for constructing tissue and organs for various significant studies. Bioink consisting of dECM from various sources is used to generate tissue-specific bioink that is unique and to mimic different biometric microenvironments. At present, there are many different techniques applied for decellularization, and the process is not standardized and regulated due to broad application. This review aims to provide an overview of different decellularization procedures, and we also emphasize the different dECM-derived bioinks present in the current global market and the major clinical outcomes. We have also highlighted an overview of benefits and limitations of different decellularization methods and various characteristic validations of decellularization and dECM-derived bioinks.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
Overview of the decellularization technique and various applications of the dECM component for in vitro studies.
Figure 2
Figure 2
Various decellularization techniques and applications of the derived dECM. Photo credits: Biorender.
Figure 3
Figure 3
Varied roles of ECM proteins.
Figure 4
Figure 4
Generalized process of generating in vitro models using dECM-derived bioinks.
See this image and copyright information in PMC

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