Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024:2849:45-54.
doi: 10.1007/7651_2024_513.

Characterization of the Newborn Epidermis and Adult Hair Follicles Using Whole-Mount Immunofluorescent Staining of Mouse Dorsal Skin

Gaia Cangiotti  1 , Anthony Veltri  1 , Georges Delepine  1 , Wen-Hui Lien  2
Affiliations

Characterization of the Newborn Epidermis and Adult Hair Follicles Using Whole-Mount Immunofluorescent Staining of Mouse Dorsal Skin

Gaia Cangiotti et al. Methods Mol Biol. 2024.

Abstract

The mammalian integumentary system, including skin and its appendages, serves as a protective barrier for the body. During development, skin epidermis undergoes rapid cell division and differentiation to form multiple stratified layers of keratinocytes. Concurrently the epidermis also gives rise to hair follicles that invaginate into the dermis. In adult skin, the hair follicle undergoes cyclic regeneration fueled by hair follicle stem cells located in the bulge. Three-dimensional and high-resolution imaging of these structures using whole-mount immunofluorescent staining allows to better characterize epidermal progenitors and stem cells.

Keywords: Confocal microscopy; Hair follicle; Hair follicle stem cells; Skin epidermis; Whole-mount immunofluorescent staining.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Blanpain C, Fuchs E (2006) Epidermal stem cells of the skin. Annu Rev Cell Dev Biol 22:339–373 - DOI - PubMed - PMC
    1. Veltri A, Lang C, Lien W-H (2018) Concise review: Wnt signaling pathways in skin development and epidermal stem cells. Stem Cells 36:22 - DOI - PubMed
    1. Millar SE (2002) Molecular mechanisms regulating hair follicle development. J Invest Dermatol 118:216–225 - DOI - PubMed
    1. Schmidt-Ullrich R, Paus R (2005) Molecular principles of hair follicle induction and morphogenesis. BioEssays 27:247–261 - DOI - PubMed
    1. Trempus CS et al (2003) Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34. J Invest Dermatol 120:501–511 - DOI - PubMed

LinkOut - more resources