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. 2024 Jun;48(11):2179-2189.
doi: 10.1007/s00266-024-03861-1. Epub 2024 Feb 26.

In Vitro Study of Thymosin Beta 4 Promoting Transplanted Fat Survival by Regulating Adipose-Derived Stem Cells

Wandi Li  1   2 , Yan Yang  3 , Yan Lin  2 , Dali Mu  4
Affiliations

In Vitro Study of Thymosin Beta 4 Promoting Transplanted Fat Survival by Regulating Adipose-Derived Stem Cells

Wandi Li et al. Aesthetic Plast Surg. 2024 Jun.

Abstract

Background: Autologous fat grafting (AFG) has emerged as a highly sought-after plastic surgery procedure, although its success has been hampered by the uncertain fat survival rate. Current evidence suggests that adipose-derived stem cells (ADSCs) may contribute to fat retention in AFG. In previous studies, it was confirmed that thymosin beta 4 (Tβ4) could enhance fat survival in vivo, although the precise mechanism remains unclear.

Methods: ADSCs were isolated from patients undergoing liposuction and their proliferation, apoptosis, anti-apoptosis, and migration were analyzed under Tβ4 stimulation using cell counting kit-8, flow cytometry, wound healing assay, and real-time quantitative PCR. The mRNA levels of genes relating to angiogenesis and Hippo signaling were also determined.

Results: Tβ4 at 100 ng/mL (p-value = 0.0171) and 1000 ng/mL (p-value = 0.0054) significantly increased ADSC proliferation from day 1 compared to the control group (0 ng/mL). In addition, the mRNA levels of proliferation-associated genes were elevated in the Tβ4 group. Furthermore, Tβ4 enhanced the anti-apoptotic ability of ADSCs when stimulated with Tβ4 and an apoptotic induction reagent (0 ng/mL vs. 1000 ng/mL, p-value = 0.011). Crucially, the mRNA expression levels of angiogenesis-related genes and critical genes in the Hippo pathway were affected by Tβ4 in ADSCs.

Conclusions: Tβ4 enhances adipose viability in AFG via facilitating ADSC proliferation and reducing apoptosis, and acts as a crucial positive regulator of ADSC-associated angiogenesis. Additionally, Tβ4 could be accountable for the phenotypic adjustment of ADSCs by regulating the Hippo pathway.

No level assigned: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Keywords: Adipose-derived stem cell; Angiogenesis; Apoptosis; Autologous fat grafting; Proliferation; Thymosin beta 4.

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References

    1. Eto H, Kato H et al (2012) The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstr Surg 129:1081–1092 - PubMed - DOI
    1. Pu LL (2016) Mechanisms of fat graft survival. Ann Plast Surg 77(Suppl 1):S84-86 - PubMed - DOI
    1. Huayllani MT, Sarabia-Estrada R et al (2020) Adipose-derived stem cells in wound healing of full-thickness skin defects: a review of the literature. J Plast Surg Hand Surg 54:263–279 - PubMed - DOI
    1. Zhang J, Liu Y et al (2020) Adipose-derived stem cells: current applications and future directions in the regeneration of multiple tissues. Stem Cells Int 2020:8810813 - PubMed - PMC - DOI
    1. Rochette L, Mazini L et al (2020) The crosstalk of adipose-derived stem cells (adsc), oxidative stress, and inflammation in protective and adaptive responses. Int J Mol Sci 21:9262 - PubMed - PMC - DOI

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