Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Dec 20;13(24):8813-26.
doi: 10.1093/nar/13.24.8813.

Molecular cloning of human apolipoprotein B cDNA

P Carlsson, S O Olofsson, G Bondjers, C Darnfors, O Wiklund, G Bjursell
Free PMC article

Molecular cloning of human apolipoprotein B cDNA

P Carlsson et al. Nucleic Acids Res. .
Free PMC article

Abstract

In this paper we describe the isolation of cDNA clones which code for parts of apolipoprotein B (apoB). The clones were obtained by immunoscreening of an expression library (lambda gt 11) derived from a human hepatoma cell line (Hep G2). The relationship between positive clones and apoB was established with immunochemical techniques using polyclonal as well as monoclonal antibodies. Recombinants, expressing nonoverlapping regions of apoB are described, all hybridizing with a very large mRNA (approximately 20,000 bases long). The nucleotide sequence obtained predicts a primary protein structure with a composition suitable for the formation of stretches of an amphipatic alpha-helix.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Cell. 1978 Oct;15(2):687-701 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Aug;82(15):4983-6 - PubMed
    1. J Biol Chem. 1979 Mar 10;254(5):1639-43 - PubMed
    1. Anal Biochem. 1979 Oct 1;98(2):305-9 - PubMed
    1. Methods Enzymol. 1980;65(1):718-49 - PubMed

Publication types