Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli
- PMID: 384145
- DOI: 10.1007/BF00268279
Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli
Abstract
Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.
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