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. 1979 May 4;172(2):171-8.
doi: 10.1007/BF00268279.

Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

M L Palmer et al. Mol Gen Genet. .

Abstract

Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.

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