Controlling arbovirus infection: high-throughput transcriptome and proteome insights

Abstract

Arboviruses pose a significant threat to public health globally, demanding innovative approaches for their control. For this, a better understanding of the complex web of interactions established in arbovirus-infected mosquitoes is fundamental. High-throughput analyses allow a genome-wide view of arbovirus-induced alterations at different gene expression levels. This review provides a comprehensive perspective into the current literature in transcriptome and proteome landscapes in mosquitoes infected with arboviruses. It also proposes a coordinated research effort to define the critical nodes that determine arbovirus infection and transmission.

Keywords: arbovirus; mosquitoes; proteome; transcriptome; vector; vector-borne diseases.

FIGURE 1

Mosquito-borne virus infection in Aedes aegypti. (1) Acquisition: viruses enter the mosquito by ingesting infected blood; (2) Dissemination: infected blood journeys to the midgut for digestion. Here, viruses must successfully infect the epithelial cells of the midgut lining to initiate infection and subsequent systemic dissemination; (3) Transmission: once viruses infect the salivary glands, they are intradermally injected together with the mosquito saliva when the mosquito bites a new host. In vivo transcriptomic (black) and proteomic (red) studies are indicated. Created with Biorender.com.