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. 2024 Feb 1;25(2):521-527.
doi: 10.31557/APJCP.2024.25.2.521.

Evaluation of HPV 16 and HPV 18 Oncoprotein Expression as Alternative Diagnostic Tools in Cervical Lesion

Nadiah Ahmad Sabri  1   2 , Shazana Hilda Shamsuddin  1 , Anani Aila Mat Zin  1   2
Affiliations

Evaluation of HPV 16 and HPV 18 Oncoprotein Expression as Alternative Diagnostic Tools in Cervical Lesion

Nadiah Ahmad Sabri et al. Asian Pac J Cancer Prev. .

Abstract

Objective: The study aimed to evaluate E6 and E7 oncoproteins of HPV16 and HPV18 expression in formalin - fixed paraffin embedded (FFPE) tissue in different grades of the cervical lesion and evaluate the potential use of E6 and E7 oncoproteins derived from HPV 16 and 18 as diagnostic protein biomarkers for triaging cervical lesions.

Methodology: A total of 102 FFPE cervical tissues were collected from 2 tertiary hospitals and immunohistochemical reactivity staining of E6 and E7 oncoproteins of HPV16 and HPV18 were evaluated using immunoreactive scoring (IRS) system and analysed statistically.

Result: The result showed an increased oncoprotein expression with the progression of cervical lesions. There is a statistically significant association between histology grade and HPV16/18-E6 expression (p = 0.028). However, there are no significant association of histological grade to HPV16-E7 immunoreactivity score (p = 0.264) and HPV18-E7 (p=0.080).

Conclusion: The immunohistochemical expression of HPV oncoproteins is a potential alternative diagnostic tool applicable in a low-resource laboratory setting. The advantage of the histochemical evaluation is that this method is simpler to apply and less expensive in comparison to in situ mRNA hybridization. Nevertheless, our study also found that antibodies against HPV that are commercially available suffer quite substantial specificity issues such as background staining and inconsistency between different batches. Hence, the utilization of antibody-based staining warrants stringent quality control.

Keywords: Cervical lesion; E6; E7; HPV16; HPV18.

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Conflict of interest statement

We have no conflicts of interest to disclose.

Figures

Figure 1
Figure 1
HPV-DNA Confirmed (by PCR) Status of Cervical Lesion
Figure 2
Figure 2
Expression of HPV Oncoproteins in the Total Cervical Lesion
Figure 3
Figure 3
Group of age based on cervical lesions
Figure 4
Figure 4
Immunohistochemical Staining Characteristic of HPV Oncoproteins in the Cervical Lesion
See this image and copyright information in PMC

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References

    1. WHO. WHO Report on Cancer Setting Priorities, Investing Wisely And Providing Care For All 2020 WHO report on cancer: setting priorities, investing wisely and providing care for all [Internet]; 2020. Available from: http://apps.Who.Int/bookorders.
    1. Azizah AM, Hashimah B, Nirmal K, Siti Zubaidah AR, Puteri NA, Nabihah A, et al. Malaysian National Cancer Registry Report 2012-2016. Putrajaya : National Cancer Institute, Ministry of Health, Malaysia; 2019.
    1. Koliopoulos G, Nyaga VN, Santesso N, Bryant A, Martin-Hirsch PP, Mustafa RA, et al. Cytology versus hpv testing for cervical cancer screening in the general population. Cochrane Database Syst Rev. 2017;8(8):Cd008587. - PMC - PubMed
    1. Liu J, Yang T, Hu Y, Ye C. The value of hpv e6/e7 mrna quantitative analysis in distinguishing high-grade cervical squamous intraepithelial lesions from low-grade cervical squamous intraepithelial lesions. J Virol Methods. 2021;289:114014. - PubMed
    1. Oyervides-Muñoz MA, Pérez-Maya AA, Rodríguez-Gutiérrez HF, Gómez-Macias GS, Fajardo-Ramírez OR, Treviño V, et al. Understanding the hpv integration and its progression to cervical cancer. Infection, Genetics and Evolution. 2018;61:134–44. - PubMed

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