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. 2024 Feb 1;25(2):547-553.
doi: 10.31557/APJCP.2024.25.2.547.

Prevalence of Hepatitis B Virus Markers among the Women with Breast Cancer

Venus Fakheri Sueini  1   2 , Mehdi Parsa Nahad  2 , Abdolhassan Talaeizadeh  3 , Maryam Moradi  4 , Amirmohammad Alborzi  1 , Roya Pirmoradi  2 , Elena Lak  5 , Manoochehr Makvandi  1
Affiliations

Prevalence of Hepatitis B Virus Markers among the Women with Breast Cancer

Venus Fakheri Sueini et al. Asian Pac J Cancer Prev. .

Abstract

Introduction: Breast cancer represents a formidable peril to the female populace on a worldwide level. The association between breast cancer and various factors, including viral infections, has been extensively investigated. Recently, the link between HBV infection and breast cancer patients has garnered attention. The present research aims to assess the prevalence of HBV markers among women diagnosed with breast cancer in Ahvaz city, Iran.

Materials and methods: Serum specimens were procured from 90 patients who had been clinically diagnosed with breast cancer. The age of the patients ranged from 29 to 80 years, with a mean age of 49.42±10.7. Histological examination of biopsy specimens revealed that 75 (83.33%) were ductal, 11 (8.88%) lobular, 2 (2.22%) mucinous, 1 (1.11%) medullary, and 1 (1.11%) was metastatic. The serum samples were subjected to initial HBsAg and anti-HBc testing via ELISA. Samples that tested seropositive (HBsAg + anti-HBc) were subsequently analyzed for the S region of HBV through nested PCR and DNA sequencing. Finally, a phylogenetic tree was constructed for positive HBV DNA tests.

Results: Among the 5/90 (5.55%) cancer patients, it was found that 3 (3.33%) cases of ductal carcinoma and one (1.11%) lobular carcinoma displayed positivity for HBV markers (HBsAg, anti-HBc, HBV PCR). Notably, one (1.11%) patient with ductal carcinoma solely demonstrated anti-HBc positivity. The phylogenetic tree analysis of the S region revealed that all HBV strains identified were categorized as genotype D.

Conclusion: The statistical analysis did not reveal any significant findings (p= 0.315) in the distribution of cancer types across different age groups. Among patients diagnosed with breast cancer, a notable prevalence of 5.5% was observed in HBV markers. The dominant HBV genotype among breast cancer patients was identified as genotype D.

Keywords: Breast; Carcinoma; Ductal; Hepatitis B Virus; Nested polymerase Chain Reaction.

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Conflict of interest statement

All authors declared that they have no conflict of interest.

Figures

Figure 1
Figure 1
A Phylogenetic Tree Using the Neighbor Joining Method was Constructed to Compare Partial Sequences of the S Region of HBV Isolates from Patients in Ahvaz City with 8 Different HBV Genotypes. The corresponding accession numbers were retrieved from GenBank, and the genotypes were isolated from various regions around the world. The constructed phylogenetic tree demonstrated the clustering of all four HBV isolates (accession numbers OR021896-OR021899) from Ahvaz city, marked with black circles, with genotype D2 from Russia (OP256019) and genotype D from Iran (JN040804). The accuracy of the phylogenetic tree was evaluated by conducting 1000 bootstrap replicates. The scale bar was set at 0.005
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