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. 1979 Jul 2;174(1):59-66.
doi: 10.1007/BF00433306.

Overlapping sequences of Klebsiella pneumoniae nifDNA cloned and characterized

F C Cannon, G E Riedel, F M Ausubel

Overlapping sequences of Klebsiella pneumoniae nifDNA cloned and characterized

F C Cannon et al. Mol Gen Genet. .

Abstract

A HindIII (17.0 kb) and an EcoR1 restriction fragment (6.9 kb) of Klebsiella pneumoniae nif DNA were cloned on two small amplifiable plasmids, pCM1 and pSA30 respectively. These plasmids between them carry 14 of the 15 known Klebsiella nif genes. The operon for the three structural genes for nitrogenase, nifpHDK, is carried on pSA30: four and five of the remaining six operons are on pCRA37 and pCM1 respectively. All of the nif genes were assigned to endonuclease restriction fragments of DNA using the Southern blotting technique (Southern, 1975) with total DNA of nif insertion mutants and radioactive plasmid DNA which contained cloned nif DNA sequences. Their locations were consistent with the genetic map of nif genes. The estimated size of the nif gene cluster was 24 kb.

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References

    1. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2866-70 - PubMed
    1. J Bacteriol. 1950 Jul;60(1):17-28 - PubMed
    1. J Bacteriol. 1978 Oct;136(1):253-66 - PubMed
    1. Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 - PubMed
    1. Basic Life Sci. 1977;9:111-28 - PubMed

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