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. 2024 Jun;47(6):e13913.
doi: 10.1111/jfd.13913. Epub 2024 Feb 29.

In vitro effects of phytogenic feed additive on Piscirickettsia salmonis growth and biofilm formation

Natacha Santibáñez  1   2 , Matías Vega  1   2 , Tatiana Pérez  1   2 , Ricardo Enriquez  1 , Carla Estefanía Escalona  1   2 , Cristian Oliver  1 , Alex Romero  1   2
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In vitro effects of phytogenic feed additive on Piscirickettsia salmonis growth and biofilm formation

Natacha Santibáñez et al. J Fish Dis. 2024 Jun.

Abstract

Piscirickettsiosis is the main cause of mortality in salmonids of commercial importance in Chile, which is caused by Piscirickettsia salmonis, a Gram-negative, γ-proteobacteria that can produce biofilm as one of its virulence factors. The Chilean salmon industry uses large amounts of antibiotics to control piscirickettsiosis outbreaks, which has raised concern about its environmental impact and the potential to induce antibiotic resistance. Thus, the use of phytogenic feed additives (PFA) with antibacterial activity emerges as an interesting alternative to antimicrobials. Our study describes the antimicrobial action of an Andrographis paniculate-extracted PFA on P. salmonis planktonic growth and biofilm formation. We observed complete inhibition of planktonic and biofilm growth with 500 and 400 μg/mL of PFA for P. salmonis LF-89 and EM-90-like strains, respectively. Furthermore, 500 μg/mL of PFA was bactericidal for both evaluated bacterial strains. Sub-inhibitory doses of PFA increase the transcript levels of stress (groEL), biofilm (pslD), and efflux pump (acrB) genes for both P. salmonis strains in planktonic and sessile conditions. In conclusion, our results demonstrate the antibacterial effect of PFA against P. salmonis in vitro, highlighting the potential of PFA as an alternative to control Piscirickettsiosis.

Keywords: Andrographis paniculata; Piscirickettsia salmonis; antimicrobial treatment; fish pathogen; phytogenic feed additive.

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REFERENCES

    1. Albornoz, R., Valenzuela, K., Pontigo, J. P., Sanchez, P., Ruiz, P., Avendano‐Herrera, R., Romero, A., Oliver, C., & Yanez, A. (2017). Identification of chemotaxis operon cheYZA and cheA gene expression under stressful conditions in Piscirickettsia salmonis. Microbial Pathogenesis, 107, 436–441.
    1. Alguel, Y., Meng, C., Teran, W., Krell, T., Ramos, J. L., Gallegos, M. T., & Zhang, X. (2007). Crystal structures of multidrug binding protein TtgR in complex with antibiotics and plant antimicrobials. Journal of Molecular Biology, 369, 829–840.
    1. Al‐Karablieh, N., Weingart, H., & Ullrich, M. S. (2009). The outer membrane protein TolC is required for phytoalexin resistance and virulence of the fire blight pathogen Erwinia amylovora. Microbial Biotechnology, 2, 465–475.
    1. Avendaño‐Herrera, R., Mancilla, M., & Miranda, C. D. (2023). Use of antimicrobials in Chilean Salmon farming: Facts, myths and perspectives. Reviews in Aquaculture, 15, 89–111.
    1. Banerjee, M., Parai, D., Chattopadhyay, S., & Mukherjee, S. K. (2017). Andrographolide: Antibacterial activity against common bacteria of human health concern and possible mechanism of action. Folia Microbiologica, 62, 237–244.

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