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. 2024 Apr 20:922:171306.
doi: 10.1016/j.scitotenv.2024.171306. Epub 2024 Feb 27.

Evaluation of Orthotrichum lyellii moss as a biomonitor of diesel exhaust

Christopher Zuidema  1 , Michael Paulsen  2 , Christopher D Simpson  2 , Sarah E Jovan  3
Affiliations

Evaluation of Orthotrichum lyellii moss as a biomonitor of diesel exhaust

Christopher Zuidema et al. Sci Total Environ. .

Abstract

Exhaust from diesel combustion engines is an important contributor to urban air pollution and poses significant risk to human health. Diesel exhaust contains a chemical class known as nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) and is enriched in 1-nitropyrene (1-NP), which has the potential to serve as a marker of diesel exhaust. The isomeric nitro-PAHs 2-nitropyrene (2-NP) and 2-nitrofluoranthene (2-NFL) are secondary pollutants arising from photochemical oxidation of pyrene and fluoranthene, respectively. Like other important air toxics, there is not extensive monitoring of nitro-PAHs, leading to gaps in knowledge about relative exposures and urban hotspots. Epiphytic moss absorbs water, nutrients, and pollutants from the atmosphere and may hold potential as an effective biomonitor for nitro-PAHs. In this study we investigate the suitability of Orthotrichum lyellii as a biomonitor of diesel exhaust by analyzing samples of the moss for 1-NP, 2-NP, and 2-NFL in the Seattle, WA metropolitan area. Samples were collected from rural parks, urban parks, residential, and commercial/industrial areas (N = 22 locations) and exhibited increasing concentrations across these land types. Sampling and laboratory method performance varied by nitro-PAH, but was generally good. We observed moderate to moderately strong correlation between 1-NP and select geographic variables, including summer normalized difference vegetation index (NDVI) within 250 m (r = -0.88, R2 = 0.77), percent impervious surface within 50 m (r = 0.83, R2 = 0.70), percent high development land use within 500 m (r = 0.77, R2 = 0.60), and distance to nearest secondary and connecting road (r = -0.75, R2 = 0.56). The relationships between 2-NP and 2-NFL and the geographic variables were generally weaker. Our results suggest O. lyellii is a promising biomonitor of diesel exhaust, specifically for 1-NP. To our knowledge this pilot study is the first to evaluate using moss concentrations of nitro-PAHs as biomonitors of diesel exhaust.

Keywords: Air toxics; Bioindicator; Epiphytic moss; Nitrated polycyclic aromatic hydrocarbons; Traffic-related air pollution; Urban air pollution.

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Conflict of interest statement

Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Christopher D. Simpson reports financial support was provided by US National Institute of Environmental Health Sciences. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure 1.
Figure 1.
Moss sampling locations in Seattle, WA, and nearby areas, covering a variety of land types.
Figure 2.
Figure 2.
Summary of nitro-PAH concentrations in moss grouped by land type (top row) and shown by individual sample (bottom row, ordered by 1-NP concentration).
Figure 3.
Figure 3.
Spatial distribution of nitro-PAH concentrations in moss in Seattle, WA and nearby areas.
Figure 4.
Figure 4.
Pearson Correlation between nitro-PAH concentrations in moss and geographic variables.
See this image and copyright information in PMC

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