Sampling and Extraction of Plant Parasitic Nematodes

Abstract

The study of nematodes requires availability of nematode specimens and their population densities in plants and soil. This can be achieved using adequate sampling schemes and extraction methods. In this chapter, the most common and suitable sampling and extraction procedures and equipment are described. These include the use of Baermann's funnels, Cobb's decanting and sieving, floating methods such as the Oostenbrink method and Fenwick can, elutriators such as Seinhorst methods, centrifugation methods including that of Coolen, and mechanical and enzymatic maceration. The combination of different methods for cleaning the nematode suspensions is described, such as Cobb's sieving with Baermann's funnels or centrifugation, and for cysts combining Seinhorst's elutriator or Fenwick can with the alcohol methods. Methods for extraction of eggs and/or juveniles of cyst and egg mass forming nematodes, to be used as inoculum or to ascertain egg viability, are also described. Only little information is also noted on the use of molecular tools to identify and quantify nematode populations in soil and roots.

Keywords: Elutriators; Extraction methods; Floating apparatus; Mechanical and enzymatic maceration; Nematode extraction; Nematode sampling.