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. 2024 Mar 3;51(1):383.
doi: 10.1007/s11033-024-09324-9.

Differential gene expression of Wharton's jelly-derived mesenchymal cells mediated by graphene oxide in basal and osteo-induced media

Vun Vun Hiew  1 , Peik Lin Teoh  2
Affiliations

Differential gene expression of Wharton's jelly-derived mesenchymal cells mediated by graphene oxide in basal and osteo-induced media

Vun Vun Hiew et al. Mol Biol Rep. .

Abstract

Background: Graphene oxide (GO) is widespread in scaffold engineering owing to its extraordinary properties such as multiple oxygen functional groups, high hydrophilicity ability and biocompatibility. It is known to promote differentiation in mesenchymal stem cells, but concomitant comparison of its modulation on the expression profiles of Wharton's jelly (WJ)-MSC surface markers, lineage differentiation, and epigenetic regulatory genes in basal and induced condition are still lacking. Unraveling the fundamental mechanisms is essential for the effective utilization of WJ-MSCs incorporated with GO in therapy. This study aims to explore the unique gene expression profiles and epigenetic characteristics of WJ-MSCs influenced by GO.

Methods and results: The characterized GO-coated coverslip served as a substrate for culturing WJ-MSCs. In addition to investigating the impact of GO on cell proliferation and differentiation, we conducted a gene expression study using PCR array, while epigenetic control was assessed through bisulfite sequencing and Western blot analysis. Our findings indicate that the presence of GO maintained the proliferation and survival of WJ-MSCs. In the absence of induction, GO led to minor lipid and glycosaminoglycan deposition in WJ-MSCs. This was evidenced by the sustained expression of pluripotency and lineage-specific genes, demethylation at the OCT4 promoter, and a decrease in H3K9 methylation. In osteo-induced condition, the occurrence of osteogenesis appeared to be guided by BMP/TGF and ERK pathway activation, accompanied by the upregulation of osteogenic-related genes and downregulation of DNMT3b.

Conclusions: GO in osteo-induced condition create a favorable microenvironment that promotes the osteogenesis of WJ-MSCs by influencing genetic and epigenetic controls. This helps in advancing our knowledge on the use of GO as priming platform and WJ-MSCs an alternate source for bone repair and regeneration.

Keywords: Chondrogenesis; Epigenetic; Gene expression; Graphene oxide; Mesenchymal stem cells; Osteogenesis.

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References

    1. Kassem DH, Kamal MM (2020) Wharton’s jelly MSCs: potential weapon to sharpen for our battle against DM. Trends Endocrinol Metab 31(4):271–273. https://doi.org/10.1016/j.tem.2020.01.001 - DOI - PubMed
    1. Ansari AS, Yazid MD, Sainik NQAV, Razali RA, Saim AB, Idrus RBH (2018) Osteogenic induction of Wharton’s jelly-derived mesenchymal stem cell for bone regeneration: a systematic review. Stem Cells Inter 2018:2406462. https://doi.org/10.1155/2018/2406462 - DOI
    1. Du Z, Wang C, Zhang R, Wang X, Li X (2020) Applications of graphene and its derivatives in bone repair: advantages for promoting bone formation and providing real-time detection, challenges and future prospects. Int J Nanomed 15:7523–7551. https://doi.org/10.2147/IJN.S271917 - DOI
    1. Li Z, Xiang S, Lin Z, Li EN, Yagi H, Cao G, Yocum L, Li L, Hao T, Bruce KK, Fritch MR, Hu H, Wang B, Alexander PG, Khor KA, Tuan RS, Lin H (2021) Graphene oxide-functionalized nanocomposites promote osteogenesis of human mesenchymal stem cells via enhancement of BMP-SMAD1/5 signaling pathway. Biomater 277:121082. https://doi.org/10.1016/j.biomaterials.2021.121082 - DOI
    1. Oliveira AML, Machado M, Silva GA, Bitoque DB, Tavares Ferreira J, Pinto LA, Ferreira Q (2022) Graphene oxide thin films with drug delivery function. Nanomaterials 12(7):1149. https://doi.org/10.3390/nano12071149 - DOI - PubMed - PMC

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