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. 2024 Feb 16:2024:10.17912/micropub.biology.001103.
doi: 10.17912/micropub.biology.001103. eCollection 2024.

Nuclear shape is affected differentially by loss of lamin A, lamin C, or both lamin A and C

Mai Pho  1 , Yasmin Berrada  1 , Aachal Gunda  1 , Andrew D Stephens  1   2
Affiliations

Nuclear shape is affected differentially by loss of lamin A, lamin C, or both lamin A and C

Mai Pho et al. MicroPubl Biol. .

Abstract

Lamin intermediate filaments form a peripheral meshwork to support nuclear shape and function. Knockout of the LMNA gene that encodes for both lamin A and C results in an abnormally shaped nucleus. To determine the relative contribution of lamin A and C to nuclear shape, we measured nuclear blebbing and circular deviation in separate lamin A and lamin C knockdown and LMNA-/- stable cells. Lamin A knockdown increased nuclear blebbing while loss of lamin A, C, or both increased circular deviation. Overall, loss of lamin A, lamin C or both lamin A/C affect nuclear shape differentially.

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Conflict of interest statement

The authors declare that there are no conflicts of interest present.

Figures

Figure 1. Nuclear blebbing and circular deviation upon loss of lamin A, C, and A/C.
Figure 1. Nuclear blebbing and circular deviation upon loss of lamin A, C, and A/C.
(A) Example images of wild type (WT), lamin A knockdown (LA KD), lamin C knockdown (LC KD), and lamin A/C knockout (LA/C KO) nuclei showing DNA (Hoechst) and lamin A and lamin C levels. (B) Representative images of normal versus blebbed nuclei. Graph of average percentage of blebbed nuclei in each condition. Three technical replicates consisting of WT 576, LA KD 323, LC KD 312, and LA/C KO 186 total cells. (C) Representative images of normal and abnormal nuclei with circular deviation values of 0.04 and 0.17, respectively. Graph of average circular deviation of nuclei in each condition. Three technical replicates consisting of WT 150, LA KD 138, LC KD 143, LA/C KO 120 total cells. Student’s t-test p values reported as * < 0.05, ** < 0.01, *** < 0.001, or no asterisk denotes no significance, p > 0.05. Error bars represent standard error. Scale bar = 10 µm.
See this image and copyright information in PMC

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