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. 2024:2849:135-148.
doi: 10.1007/7651_2024_528.

Analysis of Proliferation, Apoptosis, and Motility in Mouse Embryonic Melanocytic Precursor Cells

Melissa Crawford  1 , Kevin Barr  1 , Lina Dagnino  2   3
Affiliations

Analysis of Proliferation, Apoptosis, and Motility in Mouse Embryonic Melanocytic Precursor Cells

Melissa Crawford et al. Methods Mol Biol. 2024.

Abstract

In this chapter, we provide a method to purify and culture embryonic melanocytic stem cells that express green fluorescent protein in a cell-type specific manner. Isolation of melanocytic lineage cell populations that are >98% pure is accomplished through the use of GFP-based fluorescence activated cell sorting. We also provide a method to culture the purified melanoblasts and to analyze their proliferation, apoptosis, and motility properties.

Keywords: GFP; In vivo DNA labeling; Live cell imaging; Melanoblasts; Motility analysis; melanocytic stem cells.

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