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. 2024 Jan 26;49(1):e9.
doi: 10.5395/rde.2024.49.e9. eCollection 2024 Feb.

Pomegranate extract on eroded dentin: antioxidant action, bond strength and morphology of the adhesive interface after aging

Thiago Vinícius Cortez  1 , Nathália Mancioppi Cerqueira  1 , Julia Adornes Gallas  1 , Wanderley Pereira Oliveira  2 , Silmara Aparecida Milori Corona  1 , Aline Evangelista Souza-Gabriel  1
Affiliations

Pomegranate extract on eroded dentin: antioxidant action, bond strength and morphology of the adhesive interface after aging

Thiago Vinícius Cortez et al. Restor Dent Endod. .

Abstract

Objectives: This study aimed to evaluate the effect of pomegranate solution (Punica granatum) on eroded dentin through antioxidant action, shear bond strength (SBS) and interface morphology.

Materials and methods: The 10% pomegranate peel extract was prepared by the lyophilization method. Punicalagin polyphenol was confirmed by high-performance liquid chromatography. Antioxidant activity was evaluated by capturing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. For the SBS, 48 dentin fragments were divided into sound or eroded, and subdivided according to the pretreatment (n = 12): water or P. granatum. The surfaces were restored with self-etch adhesive and a bulk-fill resin (Ecosite; DMG). The SBS was done immediately (24 hours) and after thermal cycling + water storage (12 months). For scanning electron microscopy, 48 dentin fragments (24 sound and 24 eroded) received the same treatments as for SBS (n = 6), and they were analyzed after 24 hours and 12 months.

Results: The P. granatum had antioxidant action similar (p = 0.246) to the phenolic standard antioxidants. After 24 hours, eroded dentin had lower SBS than sound dentin (p < 0.001), regardless of the pretreatment. After 12 months, P. granatum maintained the SBS of sound dentin (13.46 ± 3.42 MPa) and eroded dentin (10.96 ± 1.90 MPa) statistically similar. The lowest values were found on eroded dentin treated with water (5.75 ± 1.65 MPa) (p < 0.001). P. granatum on eroded dentin caused peritubular demineralization and hybrid layer with resin tags.

Conclusions: The pomegranate extract had antioxidant action and preserved the adhesive interface of the eroded dentin.

Keywords: Antioxidant; Dentin-bonding agent; Punica granatum.

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Conflict of interest statement

Conflict of Interest: No potential conflict of interest relevant to this article was reported.

Figures

Figure 1
Figure 1. Failure modes after immediate bonding test and adhesive interface aging.
SBS, shear bond strength.
Figure 2
Figure 2. SEM of the interface of the restorative procedure (no degradation after the restorative procedure). (A) Sound dentin (control), (B) sound dentin + 10% P. granatum, (C) eroded dentin, and (D) eroded dentin + 10% P. granatum. Demineralized dentin without resin tags (red asterisk).
R, resin; A, adhesive; HL, hybrid layer; D, dentin; SEM, scanning electron microscopy.
Figure 3
Figure 3. SEM of the interface after degradation of the adhesive interface (12 months). (A) Sound dentin (control), (B) sound dentin + 10% P. granatum, (C) eroded dentin, (D) eroded dentin + P. granatum 10%. Demineralized dentin without resin tags (red asterisk).
R, resin; A, adhesive; HL, hybrid layer; D, dentin; SEM, scanning electron microscopy.
See this image and copyright information in PMC

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