Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Mar 8;51(1):403.
doi: 10.1007/s11033-024-09342-7.

First report of Giardia duodenalis in dairy cattle and beef cattle in Shanxi, China

Affiliations

First report of Giardia duodenalis in dairy cattle and beef cattle in Shanxi, China

Li Zhao et al. Mol Biol Rep. .

Abstract

Background: Giardia duodenalis is an important intestinal parasitic protozoan that infects several vertebrates, including humans. Cattle are considered the major source of giardiasis outbreak in humans. This study aimed to investigate the prevalence and multilocus genotype (MLG) of G. duodenalis in Shanxi, and lay the foundation for the prevention and control of Giardiosis.

Methods and results: DNA extraction, nested polymerase chain reaction, sequence analysis, MLG analysis, and statistical analysis were performed using 858 bovine fecal samples from Shanxi based on three gene loci: β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi). The overall prevalence of G. duodenalis was 28.3%, while its prevalence in Yingxian and Lingqiu was 28.1% and 28.5%, respectively. The overall prevalence of G. duodenalis in dairy cattle and beef cattle was 28.0% and 28.5%, respectively. G. duodenalis infection was detected in all age groups evaluated in this study. The overall prevalence of G. duodenalis in diarrhea and nondiarrhea samples was 32.4% and 27.5%, respectively, whereas that in intensively farmed and free-range cattle was 35.0% and 19.9%, respectively. We obtained 83, 53, and 59 sequences of bg, gdh, and tpi in G. duodenalis, respectively. Moreover, assemblage A (n = 2) and assemblage E (n = 81) by bg, assemblage A (n = 1) and assemblage E (n = 52) by gdh, and assemblage A (n = 2) and assemblage E (n = 57) by tpi were identified. Multilocus genotyping yielded 29 assemblage E MLGs, which formed 10 subgroups.

Conclusions: To the best of our knowledge, this is the first study to report cattle infected with G. duodenalis in Shanxi, China. Livestock-specific G. duodenalis assemblage E was the dominant assemblage genotype, and zoonotic sub-assemblage AI was also detected in this region.

Keywords: Giardia duodenalis; China; Multilocus genotype; Prevalence.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Santin M (2020) Cryptosporidium and Giardia in Ruminants. Vet Clin North Am Food Anim 36:223–238. https://doi.org/10.1016/j.cvfa.2019.11.005 - DOI
    1. Drake J, Sweet S, Baxendale K, Hegarty E, Horr S, Friis H, Goddu T, Ryan WG, Samson-Himmelstjerna GV (2022) Detection of Giardia and helminths in Western Europe at local K9 (canine) sites (DOGWALKS Study). Parasites Vectors 15:311. https://doi.org/10.1186/s13071-022-05440-2 - DOI - PubMed - PMC
    1. Thompson RC, Hopkins RM, Homan WL (2000) Nomenclature and genetic groupings of Giardia infecting mammals. Parasitol Today 16:210–213. https://doi.org/10.1016/S0169-4758(99)01624-5 - DOI - PubMed
    1. Zhao L, Zhang ZS, Han WX, Yang B, Chai HL, Wang MY, Wang Y, Zhang S, Zhao WH, Ma YM, Zhan YJ, Wang LF, Ding YL, Wang JL, Liu YH (2023) Prevalence and molecular characterization of Giardia duodenalis in dairy cattle in Central Inner Mongolia. Northern China Sci Rep 13:13960. https://doi.org/10.1038/s41598-023-40987-9 - DOI - PubMed
    1. Ryan U, Caccio SM (2013) Zoonotic potential of Giardia. Int J Parasitol 43:943–956 - DOI - PubMed

LinkOut - more resources