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. 2023 Dec;16(12 Suppl 2):S33-S35.

Direct Immunofluorescence

Maria R Robinson  1
Affiliations

Direct Immunofluorescence

Maria R Robinson. J Clin Aesthet Dermatol. 2023 Dec.

Abstract

Direct immunofluorescence (DIF) is a valuable diagnostic tool in the dermatology clinic. The proper use of a biopsy for DIF is dependent on several factors, including appropriate clinical indication, correct clinical site selection, and proper specimen handling and transport. Improper use of DIF can lead to false negatives, decreased diagnostic yield, and poor resource utilization. This article provides instruction on the appropriate indications and biopsy site selection for DIF. Three examples of skin diseases in which DIF would be particular useful when making a diagnosis are provided.

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Conflict of interest statement

DISCLOSURES: Dr. Robinson reports no conflicts of interest relevant to the content of this article.

Figures

Figure 1.
Figure 1.
Biopsy site selection for blistering diseases
Figure 2.
Figure 2.
Biopsy site selection for vasculitis
Figure 3.
Figure 3.
Biopsy site selection for connective tissue disease
Figure 4.
Figure 4.
Numerous erythematous bullae of varying sizes—Blue circles indicate possible biopsy locations for hematoxylin & eosin; the green circle indicates a possible biopsy location for direct immunofluorescence.
See this image and copyright information in PMC

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