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. 2024 Apr 11;13(4):e0126823.
doi: 10.1128/mra.01268-23. Epub 2024 Mar 11.

Complete genome sequence of Mycobacterium smegmatis phage Rummer, a subcluster A3 actinophage

Dondra S Bailey  1 , Dominique R Dotson  1 , Charlotte Berkes  2 , Oluwanifemi Agbede  1 , Marcelaine Augustin  1 , Imani Blackman-Murray  1 , Talaeya Chambers  1 , Nicolas Felber  1 , Dy'Mon Fleming  1 , Loretta Frazier  1 , Natalie Gray  1 , Ayanna Harrison  1 , Genesis Hernandez  1 , Nina Iwuchukwu  1 , Chika Iwuji  1 , Taysha Jackson  1 , Angelic Jefferson  1 , Daya Jordan  1 , Miracle Jordan  1 , Brian Nicolas  1 , Monae Person  1 , Ga'Nayah Richardson  1 , Ashley Roman  1 , Christian Stevens  1 , My'Sean Suggs  1 , Nahshon Thompson  1 , Summer Timmons-Smith  1 , Shiaishea Wilfong  1 , Micaela Wilson-Wheatley  1
Affiliations

Complete genome sequence of Mycobacterium smegmatis phage Rummer, a subcluster A3 actinophage

Dondra S Bailey et al. Microbiol Resour Announc. .

Abstract

Bacteriophage Rummer is a siphovirus morphology actinophage isolated from Mycobacterium smegmatis. Rummer has a 50,908 base pair genome encoding 89 predicted protein-coding genes and three tRNAs. Based on gene content similarity to sequenced actinobacteriophages, Rummer is assigned to phage subcluster A3.

Keywords: actinophage; bacteriophage.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig 1
Fig 1
Characterization of Rummer. (A) Plaque morphology. Plaques produced on a lawn of M. smegmatis PYCa medium on a petri dish (90 mm diameter). Plaques are with a clear center surrounded by a turbid edge. (B) Negative-stain (1% uranyl acetate) transmission electron micrograph of Rummer. Phage particles were analyzed using JEOL JEM-1400 Transmission Electron Microscope at an accelerating voltage of 80 kV. Rummer has a siphoviral morphology with a capsid diameter of 50 nm and a tail length of 160 nm (n = 1).
See this image and copyright information in PMC

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