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. 2024 May:190:106612.
doi: 10.1016/j.micpath.2024.106612. Epub 2024 Mar 11.

Isolation and characterization of a G9P[23] porcine rotavirus strain AHFY2022 in China

Jianxin Wang  1 , Jinzhu Zhou  2 , Xuejiao Zhu  2 , Xianyu Bian  3 , Nan Han  3 , Baochao Fan  2 , Laqiang Gu  3 , Xi Cheng  3 , Sufen Li  3 , Ran Tao  2 , Jizong Li  2 , Xuehan Zhang  4 , Bin Li  5
Affiliations

Isolation and characterization of a G9P[23] porcine rotavirus strain AHFY2022 in China

Jianxin Wang et al. Microb Pathog. 2024 May.

Abstract

Rotavirus group A (RVA) is a main pathogen causing diarrheal diseases in humans and animals. Various genotypes are prevalent in the Chinese pig herd. The genetic diversity of RVA lead to distinctly characteristics. In the present study, a porcine RVA strain, named AHFY2022, was successfully isolated from the small intestine tissue of piglets with severe diarrhea. The AHFY2022 strain was identified by cytopathic effects (CPE) observation, indirect immunofluorescence assay (IFA), electron microscopy (EM), high-throughput sequencing, and pathogenesis to piglets. The genomic investigation using NGS data revealed that AHFY2022 exhibited the genotypes G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1, using the online platform the Bacterial and Viral Bioinformatics Resource Center (BV-BRC) (https://www.bv-brc.org/). Moreover, experimental inoculation in 5-day-old and 27-day-old piglets demonstrated that AHFY2022 caused severe diarrhea, fecal shedding, small intestinal villi damage, and colonization in all challenged piglets. Taken together, our results detailed the virological features of the porcine rotavirus G9P[23] from China, including the whole-genome sequences, genotypes, growth kinetics in MA104 cells and the pathogenicity in suckling piglets.

Keywords: Genome analysis; Isolation; Pathogenicity; Porcine rotavirus; Whole-genome sequencing.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no know competing financial interests or personal relationships that could have appeared to influence the work reported in this manuscript. The manuscript is approved by all authors for publication. I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. All the authors listed have approved the manuscript that is enclosed.

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