Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2024 Mar 1:22:101267.
doi: 10.1016/j.fochx.2024.101267. eCollection 2024 Jun 30.

Flow field-flow fractionation coupled with multidetector: A robust approach for the separation and characterization of resistant starch

Mu Wang  1 , Wenhui Zhang  1 , Liu Yang  1 , Yueqiu Li  1 , Hailiang Zheng  2 , Haiyang Dou  1   3   4
Affiliations
Review

Flow field-flow fractionation coupled with multidetector: A robust approach for the separation and characterization of resistant starch

Mu Wang et al. Food Chem X. .

Abstract

The unique properties of resistant starch (RS) have made it applicable in the formulation of a broad range of functional foods. The physicochemical properties of RS play a crucial role in its applications. Recently, flow field-flow fractionation (FlFFF) has attracted increasing interest in the separation and characterization of different categories of RS. In this review, an overview of the theory behind FlFFF is introduced, and the controllable factors, including FlFFF channel design, sample separation conditions, and the choice of detector, are discussed in detail. Furthermore, the applications of FlFFF for the separation and characterization of RS at both the granule and molecule levels are critically reviewed. The aim of this review is to equip readers with a fundamental understanding of the theoretical principle of FlFFF and to highlight the potential for expanding the application of RS through the valuable insights gained from FlFFF coupled with multidetector analysis.

Keywords: Characterization; Flow field-flow fractionation; Resistant starch.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Classification and application of RS.
Fig. 2
Fig. 2
Schematic diagram of the channel (a and b) and separation process (c) of FlFFF.
Fig. 3
Fig. 3
Main separation modes of FlFFF.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Arfvidsson C., Wahlund K.G. Mass overloading in the flow field-flow fractionation channel studied by the behaviour of the ultra-large wheat protein glutenin. Journal of Chromatography A. 2003;1011(1–2):99–109. doi: 10.1016/S0021-9673(03)01145-2. - DOI - PubMed
    1. Asp N.G. Resistant starch. Proceedings for the 2nd plenary meeting of EURESTA: European FLAIR Concerted Action No. 11 on physiological implications of the consumption of resistant starch in man. European Journal of Clinical Nutrition. 1992;38:S1–S148. doi: 10.1021/ac00176a004. - DOI - PubMed
    1. Bello-Perez L.A., Agama-Acevedo E., Lopez-Silva M., Alvarez-Ramirez J. Molecular characterization of corn starches by HPSEC-MALS-RI: A comparison with AF4-MALS-RI system. Food Hydrocolloids. 2019;96:373–376. doi: 10.1016/j.foodhyd.2019.04.067. - DOI
    1. Benincasa M.A., Giddings J.C. Separation and molecular weight distribution of anionic and cationic water-soluble polymers by flow field-flow fractionation. Analytical Chemistry. 1992;64(7):790–798. doi: 10.1021/ac00031a016. - DOI
    1. Brown I. Complex carbohydrates and resistant starch. Nutrition Reviews. 1996;54(11):S115–S119. doi: 10.1111/j.1753-4887.1996.tb03830.x. - DOI - PubMed

LinkOut - more resources