Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Mar 12;40(4):132.
doi: 10.1007/s11274-024-03947-7.

Lactococcus garvieae exerts a critical role in inducing inflammation in dairy mastitis by triggering NLRP3 inflammasome-mediated pyroptosis in MAC-T cells

Fan Wu  1 , Tao Du  1 , Xiaodan Jiang  1 , Shuhong Liu  1 , Yiru Cheng  1 , Zhe Zhang  1 , Wei Miao  2 , Tiancheng Wang  3
Affiliations

Lactococcus garvieae exerts a critical role in inducing inflammation in dairy mastitis by triggering NLRP3 inflammasome-mediated pyroptosis in MAC-T cells

Fan Wu et al. World J Microbiol Biotechnol. .

Abstract

Lactococcus garvieae (L. garvieae) is a pathogenic bacterium that is Gram-positive and catalase-negative (GPCN), and it is capable of growing in a wide range of environmental conditions. This bacterium is associated with significant mortality and losses in fisheries, and there are concerns regarding its potential as a zoonotic pathogen, given its presence in cattle and dairy products. While we have identified and characterized virulent strains of L. garvieae through phenotyping and molecular typing studies, their impact on mammary tissue remains unknown. This study aims to investigate the pathogenicity of strong and weak virulent strains of L. garvieae using in vivo mouse models. We aim to establish MAC-T cell model to examine potential injury caused by the strong virulent strain LG41 through the TLR2/NLRP3/NF-kB pathway. Furthermore, we assess the involvement of NLRP3 inflammasome-mediated pyroptosis in dairy mastitis by silencing NLRP3. The outcomes of this study will yield crucial theoretical insights into the potential mechanisms involved in mastitis in cows caused by the L. garvieae-induced inflammatory response in MAC-T cells.

Keywords: Lactococcus garvieae; Bovine mastitis; Inflammatory; NLRP3; Pyroptosis.

PubMed Disclaimer

References

    1. Acharya KR, Brankston G, Slavic D, Greer AL (2021) Spatio-temporal variation in the prevalence of major mastitis pathogens isolated from bovine milk samples between 2008 and 2017 in Ontario, Canada. Front Vet Sci 8:742696. https://doi.org/10.3389/fvets.2021.742696 - DOI - PubMed - PMC
    1. Aitken SL, Corl CM, Sordillo LM (2011) Pro-inflammatory and pro-apoptotic responses of TNF-α stimulated bovine mammary endothelial cells. Vet Immunol Immunopathol 140(3–4):282–290. https://doi.org/10.1016/j.vetimm.2011.01.016 - DOI - PubMed
    1. Akhtar M, Guo S, Guo YF, Zahoor A, Shaukat A, Chen Y, Umar T, Deng PG, Guo M (2020) Upregulated-gene expression of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) via TLRs following NF-κB and MAPKs in bovine mastitis. Acta Trop 207:105458. https://doi.org/10.1016/j.actatropica.2020.105458 - DOI - PubMed
    1. Barkema HW, von Keyserlingk MA, Kastelic JP, Lam TJ, Luby C, Roy JP, LeBlanc SJ, Keefe GP, Kelton DF (2015) Invited review: changes in the dairy industry affecting dairy cattle health and welfare. J Dairy Sci 98(11):7426–7445. https://doi.org/10.3168/jds.2015-9377 - DOI - PubMed
    1. Bauernfeind FG, Horvath G, Stutz A, Alnemri ES, MacDonald K, Speert D, Fernandes-Alnemri T, Wu J, Monks BG, Fitzgerald KA, Hornung V, Latz E (2009) Cutting edge: NF-kappaB activating pattern recognition and cytokine receptors license NLRP3 inflammasome activation by regulating NLRP3 expression. J Immunol 183(2):787–791. https://doi.org/10.4049/jimmunol.0901363 - DOI - PubMed

Substances

Supplementary concepts

LinkOut - more resources