Optical Sensor System for 3D Jones Matrix Reconstruction of Optical Anisotropy Maps of Self-Assembled Polycrystalline Soft Matter Films

Abstract

Our work uses a polarization matrix formalism to analyze and algorithmically represent optical anisotropy by open dehydration of blood plasma films. Analytical relations for Jones matrix reconstruction of optical birefringence maps of protein crystal networks of dehydrated biofluid films are found. A technique for 3D step-by-step measurement of the distributions of the elements of the Jones matrix or Jones matrix images (JMI) of the optically birefringent structure of blood plasma films (BPF) has been created. Correlation between JMI maps and corresponding birefringence images of dehydrated BPF and saliva films (SF) obtained from donors and prostate cancer patients was determined. Within the framework of statistical analysis of layer-by-layer optical birefringence maps, the parameters most sensitive to pathological changes in the structure of dehydrated films were found to be the central statistical moments of the 1st to 4th orders. We physically substantiated and experimentally determined the sensitivity of the method of 3D polarization scanning technique of BPF and SF preparations in the diagnosis of endometriosis of uterine tissue.

Keywords: 3D reconstruction; Jones matrix; blood plasma; diagnostics; optical anisotropy.

Figure 1

Optical scheme of optical sensor system for Jones matrix tomography of polycrystalline films of biological fluids. 1—HeNe laser (Ø = 2 × 10³ μm, λ = 0.6328 μm); 2—collimator; 3—light splitter (50%); 4—turning mirror; 5, 7, 10, 12, 13—linear polarizers; 6, 11—quarter wave plates; 8—sample of dehydrated film; 9—polarizing microobjective; 14—digital camera; 15—PC.

Figure 2

Topographic maps of linear ${\theta }_{blood}^{\mathrm{*}}\left(LB\right)=0.35\mathrm{rad}$ (Fragments (1), (2)) and circular ${\theta }_{blood}^{\mathrm{*}}\left(CB\right)=0.25\mathrm{rad}$ (Fragments (3), (4)) birefringence of healthy donors BPF (Fragments (1), (3)) and sick patients (Fragments (2), (4)).

Figure 3

Topographic maps of linear ${\theta }_{blood}^{\mathrm{*}}\left(LB\right)=0.35\mathrm{rad}$ (Fragments (1), (2)) and circular ${\theta }_{blood}^{\mathrm{*}}\left(CB\right)=0.25\mathrm{rad}$ (Fragments (3), (4)) birefringence of SF of healthy donors (Fragments (1), (3)) and sick patients (Fragments (2), (4)).